0 556

Cited 119 times in

Functional recovery after human umbilical cord blood cells transplantation with brain-derived neutrophic factor into the spinal cord injured rat

 S.-U. Kuh  ;  Y.-E. Cho  ;  D.-H. Yoon  ;  K.-N. Kim  ;  Y. Ha 
 ACTA NEUROCHIRURGICA, Vol.147(9) : 985-992, 2005 
Journal Title
Issue Date
Animals ; Biomarkers/metabolism ; Brain Stem/drug effects ; Brain Stem/physiology ; Brain-Derived Neurotrophic Factor/pharmacology* ; Brain-Derived Neurotrophic Factor/therapeutic use ; Cell Differentiation/drug effects ; Cell Differentiation/physiology ; Cell Proliferation/drug effects ; Cells, Cultured ; Cord Blood Stem Cell Transplantation/methods* ; Cord Blood Stem Cell Transplantation/trends ; Efferent Pathways/drug effects ; Efferent Pathways/physiology ; Glial Fibrillary Acidic Protein/metabolism ; Graft Survival/drug effects ; Graft Survival/physiology ; Growth Cones/drug effects ; Growth Cones/physiology ; Humans ; Male ; Microtubule-Associated Proteins/metabolism ; Nerve Regeneration/drug effects ; Nerve Regeneration/physiology ; Neuroglia/cytology ; Neuroglia/drug effects ; Neuroglia/metabolism ; Neurons/cytology ; Neurons/drug effects ; Neurons/metabolism ; Pluripotent Stem Cells/drug effects ; Pluripotent Stem Cells/physiology* ; Pluripotent Stem Cells/transplantation* ; Rats ; Rats, Sprague-Dawley ; Recovery of Function/drug effects ; Recovery of Function/physiology* ; Spinal Cord Injuries/physiopathology ; Spinal Cord Injuries/therapy* ; Transplantation, Heterologous ; Treatment Outcome
Human umbilical cord blood cells (HUCBs) ; cell transplantation ; spinal cord injury ; BDNF
There have been many efforts to recover neuronal function from spinal cord injuries, but there are some limitations in the treatment of spinal cord injuries.
The neural stem cell has been noted for its pluripotency to differentiate into various neural cell types. The human umbilical cord blood cells (HUCBs) are more pluripotent and genetically flexible than bone marrow neural stem cells. The HUCBs could be more frequently used for spinal cord injury treatment in the future.
Moderate degree spinal cord injured rats were classified into 3 subgroups, group A: media was injected into the cord injury site, group B: HUCBs were transplanted into the cord injury site, and group C: HUCBs with BDNF (Brain-derived neutrophic factor) were transplanted into the cord injury site. We checked the BBB scores to evaluate the functional recovery in each group at 8 weeks after transplantation. We then, finally checked the neural cell differentiation with double immunofluorescence staining, and we also analyzed the axonal regeneration with retrograde labelling of brain stem neurons by using fluorogold. The HUCBs transplanted group improved, more than the control group at every week after transplantation, and also, the BDNF enabled an improvement of the BBB locomotion scores since the 1 week after its application (P<0.05). 8 weeks after transplantation, the HUCBs with BDNF transplanted group had more greatly improved BBB scores, than the other groups (P<0.001). The transplanted HUCBs were differentiated into various neural cells, which were confirmed by double immunoflorescence staining of BrdU and GFAP & MAP-2 staining. The HUCBs and BDNF each have individual positive effects on axonal regeneration. The HUCBs can differentiate into neural cells and induce motor function improvement in the cord injured rat models. Especially, the BDNF has effectiveness for neurological function improvement due to axonal regeneration in the early cord injury stage. Thus the HUCBs and BDNF have recovery effects of a moderate degree for cord injured rats.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kuh, Sung Uk(구성욱) ORCID logo https://orcid.org/0000-0003-2566-3209
Kim, Keung Nyun(김긍년)
Yoon, Do Heum(윤도흠) ORCID logo https://orcid.org/0000-0003-1452-5724
Cho, Yong Eun(조용은) ORCID logo https://orcid.org/0000-0001-9815-2720
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.