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Proteomic analysis of progressive factors in uterine cervical cancer

Authors
 Yoon Pyo Choi  ;  Suki Kang  ;  Sunghee Hong  ;  Xianhe Xie  ;  Nam Hoon Cho 
Citation
 PROTEOMICS, Vol.5(6) : 1481-1493, 2005 
Journal Title
 PROTEOMICS 
ISSN
 1615-9853 
Issue Date
2005
MeSH
Differential expression protein ; Human uterine cervical cancer ; Matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry ; Two‐dimensional gel electrophoresis
Keywords
Differential expression protein ; Human uterine cervical cancer ; Matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry ; Two‐dimensional gel electrophoresis
Abstract
Human papillomavirus (HPV) infections play a crucial role in the progress of cervical cancer. The high-risk HPV types are frequently associated with the development of malignant lesions. Some of the latest studies have demonstrated that the high-risk HPV 16 and 18 are predominantly detected in the more aggressive cancers. In the present study, we aimed to establish the proteomic profiles and characterization of the tumor related proteins by using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). For proteomic analysis, patients infected by HPV 16 or 18 were included in this study. We compared nuclear protein and cytoplasmic protein, separately by using the subcellular fraction. Differential protein spots between cervical cancer with high-risk HPV, HPV 16 or HPV 18, and HaCaT cell lines were characterized by 2-DE. Those proteins analyzed by peptide mass fingerprinting based on MALDI-TOF MS and database searching were the products of oncogenes or proto-oncogenes, and the others were involved in the regulation of cell cycle, for general genomic stability, telomerase activation, and cell immortalization. However, there was no difference in protein characterization for cervical cancer between HPV 16 and HPV 18 infection. Nonetheless, these data are valuable for the mass identification of differentially expressed proteins involved in human uterine cervical cancer. Moreover, the data has enormous value for establishing the human uterine cervical cancer proteome database that can be used in screening a molecular marker for the further study of human uterine cervical cancer, and also for studying any correlation among the cancers induced by HPV.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/pmic.200401021/abstract
DOI
10.1002/pmic.200401021
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kang, Suki(강숙희) ORCID logo https://orcid.org/0000-0002-9957-3479
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/150832
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