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Implantation of bone marrow mononuclear cells using injectable fibrin matrix enhances neovascularization in infarcted myocardium

 Ju Hee Ryu  ;  Il-Kwon Kim  ;  Seung-Woo Cho  ;  Myeong-Chan Cho  ;  Kyung-Kuk Hwang  ;  Hainan Piao  ;  Shuguang Piao  ;  Sang Hyun Lim  ;  Yoo Sun Hong  ;  Cha Yong Choi  ;  Kyung Jong Yoo  ;  Byung-Soo Kim 
 BIOMATERIALS, Vol.26(3) : 319-326, 2005 
Journal Title
Issue Date
Animals ; Biomimetic Materials/chemistry ; Bone Marrow Transplantation/methods* ; Cell Culture Techniques/methods* ; Cell Survival ; Extracellular Matrix/chemistry ; Extracellular Matrix/physiology ; Fibrin/administration & dosage* ; Injections ; Microcirculation/pathology ; Microcirculation/physiopathology ; Monocytes/pathology* ; Monocytes/transplantation* ; Myocardial Infarction/pathology* ; Myocardial Infarction/surgery* ; Neovascularization, Physiologic/physiology* ; Rats ; Rats, Sprague-Dawley ; Treatment Outcome
Fibrin marix ; Bone marrow mononuclear cells ; Myocardial infarction ; Neovascularization
Neovascularization may improve cardiac function and prevent further scar tissue formation in infarcted myocardium. A number of studies have demonstrated that bone marrow-derived cells have the potential to induce neovascularization in ischemic tissues. In this study, we hypothesized that implantation of bone marrow mononuclear cells (BMMNCs) using injectable fibrin matrix further enhances neovascularization in infarcted myocardium compared to BMMNC implantation without matrix. To test this hypothesis, infarction was induced in rat myocardium by cryoinjury. Three weeks later, rat BMMNCs were mixed with fibrin matrix and injected into the infarcted myocardium. Injection of either BMMNCs or medium alone into infarcted myocardium served as controls. Eight weeks after the treatments, histological analyses indicated that implantation of BMMNCs using fibrin matrix resulted in more extensive tissue regeneration in the infarcted myocardium compared to BMMNC implantation without matrix. Examination with fluorescence microscopy revealed that cells labeled with a fluorescent dye prior to implantation survived in the infarcted myocardium at 8 weeks of implantation. Importantly, implantation of BMMNCs using fibrin matrix resulted in much more extensive neovascularization in infarcted myocardium than BMMNC implantation without matrix. The microvessel density in infarcted myocardium was significantly higher (p<0.05) when BMMNCs were implanted using fibrin matrix (350±22 microvessels/mm2) compared to BMMNC implantation without matrix (262±13 microvessels/mm2) and medium injection (76±9 microvessels/mm2). In addition, average internal diameter of microvessels was significantly larger (p<0.05) in BMMNC implantation with fibrin matrix group (14.6±1.2 μm) than BMMNC implantation without matrix group (10.2±0.7 μm) and medium injection group (7.3±0.5 μm). These results suggest that fibrin matrix could serve as a cell implantation matrix that enhances neovascularization efficacy for myocardial infarction treatment.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Il Kwon(김일권)
Yoo, Kyung Jong(유경종) ORCID logo https://orcid.org/0000-0002-9858-140X
Lim, Sang Hyun(임상현)
Hong, You Sun(홍유선)
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