Cited 24 times in
Peroxynitrite modulates release of inflammatory mediators from guinea pig lung mast cells activated by antigen-antibody reaction
DC Field | Value | Language |
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dc.contributor.author | 노재열 | - |
dc.contributor.author | 이광훈 | - |
dc.contributor.author | 이봉기 | - |
dc.date.accessioned | 2017-10-26T06:12:53Z | - |
dc.date.available | 2017-10-26T06:12:53Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1018-2438 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/150661 | - |
dc.description.abstract | BACKGROUND: Peroxynitrite (ONOO-), the product of the reaction between the superoxide anion (*O2-) and nitric oxide (NO), is produced during inflammatory disease and may be a major cytotoxic agent. No reports are available as to whether ONOO- generates or modulates inflammatory mediator release from activated guinea pig lung mast cells. In this study, we explored the modulatory role of intracellular ONOO- on inflammatory mediator release (histamine and leukotrienes) from activated mast cells. METHODS: Guinea pig lung mast cells were purified by the enzyme digestion, and by using the rough and discontinuous Percoll density gradients. Mast cells were sensitized with IgG1 (anti-ovalbumin) antibody and challenged with ovalbumin (OVA). The intracellular ROS formation was determined by following the oxidative production of 2', 7'-dichlorofluorescein diacetate (DCFH-DA), dihydrorhodamine 123 (DHR), and anti-nitrotyrosine antibody immunofluorescence. Histamine was assayed using a fluorometric analyzer, leukotrienes by radioimmunoassay, intracellular Ca2+ levels by confocal scanning microscopy, and PLA(2) activity using prelabeling of [3H]arachidonic acid. RESULTS: ROS detected by DCFH-DA weakly increased in mast cells activated with OVA (1.0 g/ml), and the ROS so generated was inhibited by ebselen (50 microM). However, the ROS detected by DHR increased 3-fold under the same conditions. Peroxynitrite scavengers sL-MT, DMTU, and inhibitor FeTPPS inhibited ROS formation but the NADPH oxidase inhibitor diphenyleneiodonium (DPI) only partially inhibited this formation. Dimethyl thiourea (DMTU) and seleno-L-methionine (sL-MT) inhibited the tyrosine nitration of cytosolic proteins, the release of histamine and leukotrienes, Ca2+ influx, and the PLA(2) activity evoked by mast cell activation. CONCLUSION: The data obtained suggests that the ROS generated by the antigen/antibody reaction activated mast cells is ONOO-, and that this modulates the release of inflammatory mediators via Ca2+ -dependent PLA(2) activity. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | S. Karger | - |
dc.relation.isPartOf | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigen-Antibody Reactions | - |
dc.subject.MESH | Antioxidants/pharmacology | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Guinea Pigs | - |
dc.subject.MESH | Histamine Release* | - |
dc.subject.MESH | Leukotrienes/metabolism* | - |
dc.subject.MESH | Lung/cytology | - |
dc.subject.MESH | Lung/immunology* | - |
dc.subject.MESH | Mast Cells/immunology* | - |
dc.subject.MESH | Nitric Oxide/metabolism | - |
dc.subject.MESH | Peroxynitrous Acid/physiology* | - |
dc.subject.MESH | Phospholipases A/metabolism | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | Tyrosine/analysis | - |
dc.title | Peroxynitrite modulates release of inflammatory mediators from guinea pig lung mast cells activated by antigen-antibody reaction | - |
dc.type | Article | - |
dc.publisher.location | Switzerland | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Ji Young Kim | - |
dc.contributor.googleauthor | Kwang Hoon Lee | - |
dc.contributor.googleauthor | Bong Ki Lee | - |
dc.contributor.googleauthor | Jai Youl Ro | - |
dc.identifier.doi | 10.1159/000085465 | - |
dc.contributor.localId | A01291 | - |
dc.contributor.localId | A02674 | - |
dc.contributor.localId | A02806 | - |
dc.relation.journalcode | J01074 | - |
dc.identifier.eissn | 1423-0097 | - |
dc.identifier.pmid | 15855792 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/85465 | - |
dc.subject.keyword | Mast cells | - |
dc.subject.keyword | Peroxynitrite | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.subject.keyword | Antioxidants | - |
dc.subject.keyword | Intracellular Ca2+ level | - |
dc.subject.keyword | Phospholipase A2 | - |
dc.contributor.alternativeName | Ro, Jai Youl | - |
dc.contributor.alternativeName | Lee, Kwang Hoon | - |
dc.contributor.alternativeName | Lee, Bong Ki | - |
dc.citation.volume | 137 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 104 | - |
dc.citation.endPage | 114 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.137(2) : 104-114, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 44222 | - |
dc.type.rims | ART | - |
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