0 437

Cited 9 times in

Antiangiogenic Effect of ZD1839 against Murine Renal Cell Carcinoma (RENCA) in an Orthotopic Mouse Model

Authors
 Oh H.Y.  ;  Kwon S.M.  ;  Kim S.  ;  Jae Y.W.  ;  Hong S.J. 
Citation
 UROLOGIA INTERNATIONALIS, Vol.75(2) : 159-166, 2005 
Journal Title
UROLOGIA INTERNATIONALIS
ISSN
 0042-1138 
Issue Date
2005
MeSH
Animals ; Biopsy, Needle ; Blotting, Western ; Carcinoma, Renal Cell/drug therapy ; Carcinoma, Renal Cell/pathology ; Cell Proliferation/drug effects ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; ErbB Receptors/analysis* ; Female ; Gefitinib ; Immunohistochemistry ; Kidney Neoplasms/drug therapy ; Kidney Neoplasms/pathology ; Mice ; Mice, Inbred BALB C ; Neovascularization, Pathologic/prevention & control* ; Quinazolines/pharmacology* ; Sensitivity and Specificity ; Transplantation, Heterologous ; Tumor Cells, Cultured/cytology ; Tumor Cells, Cultured/drug effects*
Keywords
16123571
Abstract
Introduction: ZD1839 (IressaTM) is a selective epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI). We evaluated the antitumor and antiangiogenesis activities of ZD1839 in a murine renal cell carcinoma (RENCA) model.
Materials and Methods: The effect of ZD1839 on the cellular proliferation of RENCA cells in vitro was measured by colorimetric assay. For the in vivo studies, RENCA cells were adsorbed in Gelfoam and implanted into BALB/cJ mouse parenchyma with an agarose bar. Mice were treated with ZD1839 (40 mg/kg/day s.c.), genistein or saline for 14 days. Western blot analysis was performed to observe EGFR expression in RENCA cells and tumor tissues. Microvessel density (MVD) was quantified by immunostaining for factor VIII-related antigens and VEGF level was assayed by ELISA.
Results: ZD1839 showed a dose-dependent inhibition of RENCA cellular proliferation. ZD1839 treatment resulted in a marked decrease in tumor growth compared with saline treatment. The MVD and VEGF in the RENCA tumors were decreased significantly by ZD1839 (p < 0.01 and p >0.05, respectively). Genistein also suppressed tumor growth and decreased MVD and VEGF level, but the efficacies were less than with ZD1839.
Conclusion: The suppressive activity of ZD1839 on RENCA tumor growth was accompanied by decreases in the MVD and VEGF production. These results suggest that the antitumor effect of ZD1839 in a RENCA model is mediated partially by the inhibition of tumor angiogenesis.
Full Text
http://www.karger.com/Article/FullText/87171
DOI
10.1159/000087171
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kwon, Soo Mee(권수미)
Yang, Won Jae(양원재)
Oh, Hea Young(오혜영)
Hong, Sung Joon(홍성준) ORCID logo https://orcid.org/0000-0001-9869-065X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/150522
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links