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Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter

Authors
 Seung-Soon Im  ;  Jae-Woo Kim  ;  Tae-Hyun Kim  ;  Xian-Li Song  ;  So-Youn Kim  ;  Ha-Il Kim  ;  Yong-Ho Ahn 
Citation
 Experimental and Molecular Medicine, Vol.37(2) : 101-110, 2005 
Journal Title
 Experimental and Molecular Medicine 
ISSN
 1226-3613 
Issue Date
2005
MeSH
Animals ; Cells, Cultured ; Chromatin Immunoprecipitation ; Gene Expression Regulation ; Genes, Reporter ; Glucose Transporter Type 2 ; Hepatocytes/metabolism* ; Liver/metabolism ; Male ; Mice ; Mice, Inbred ICR ; Mice, Transgenic ; Monosaccharide Transport Proteins/biosynthesis* ; Monosaccharide Transport Proteins/genetics ; Mutagenesis, Site-Directed ; PPAR alpha/genetics ; PPAR alpha/metabolism ; PPAR gamma/agonists ; PPAR gamma/genetics ; PPAR gamma/metabolism* ; Promoter Regions, Genetic* ; Protein Isoforms/biosynthesis ; Response Elements* ; Rosiglitazone ; Thiazolidinediones/pharmacology
Keywords
15886523
Abstract
In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-γ in the liver. Rosiglitazone, PPAR-γ agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-γ/RXR-α. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-γ rather than PPAR-α binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-γ ligand contributing to glucose transport into liver in a condition when PAPR-γ expression is increased as in type 2 diabetes or in severe obesity.
Files in This Item:
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DOI
10.1038/emm.2005.14
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, So Youn(김소연)
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
Kim, Tae Hyun(김태현)
Kim, Ha Il(김하일)
Song, Xian Li(송현려)
Ahn, Yong Ho(안용호) ORCID logo https://orcid.org/0000-0002-4133-0757
Im, Seung Soon(임승순)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/150036
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