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FBI-1 Enhances Transcription of the Nuclear Factor-κB (NF-κB)-responsive E-selectin Gene by Nuclear Localization of the p65 Subunit of NF-κB

 Dong-Kee Lee  ;  Jae-Eun Kang  ;  Hye-Jin Park  ;  Myung-Hwa Kim  ;  Tae-Hee Yim  ;  Jung-Min Kim  ;  Min-Kyu Heo  ;  Kyu-Yeun Kim  ;  Ho Jeong Kwon  ;  Man-Wook Hur 
 JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.280(30) : 27783-27791, 2005 
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The POZ domain is a highly conserved protein-protein interaction motif found in many regulatory proteins. Nuclear factor-kappaB (NF-kappaB) plays a key role in the expression of a variety of genes in response to infection, inflammation, and stressful conditions. We found that the POZ domain of FBI-1 (factor that binds to the inducer of short transcripts of human immunodeficiency virus-1) interacted with the Rel homology domain of the p65 subunit of NF-kappaB in both in vivo and in vitro protein-protein interaction assays. FBI-1 enhanced NF-kappaB-mediated transcription of E-selectin genes in HeLa cells upon phorbol 12-myristate 13-acetate stimulation and overcame gene repression by IkappaB alpha or IkappaB beta. In contrast, the POZ domain of FBI-1, which is a dominant-negative form of FBI-1, repressed NF-kappaB-mediated transcription, and the repression was cooperative with IkappaB alpha or IkappaB beta. In contrast, the POZ domain tagged with a nuclear localization sequence polypeptide of FBI-1 enhanced NF-kappaB-responsive gene transcription, suggesting that the molecular interaction between the POZ domain and the Rel homology domain of p65 and the nuclear localization by the nuclear localization sequence are important in the transcription enhancement mediated by FBI-1. Confocal microscopy showed that FBI-1 increased NF-kappaB movement into the nucleus and increased the stability of NF-kappaB in the nucleus, which enhanced NF-kappaB-mediated transcription of the E-selectin gene. FBI-1 also interacted with IkappaB alpha and IkappaB beta.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myung Hwa(김명화)
Kim, Jung Min(김정민)
Park, Hye Jin(박혜진)
Lee, Dong Kee(이동기)
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
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