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영구적 국소 대뇌 허혈 생쥐 모델에서 활성 산소종에 의한 세포고사 유도인자의 핵내 이동과 세포고사
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김경환 | - |
dc.contributor.author | 김현우 | - |
dc.contributor.author | 이병인 | - |
dc.contributor.author | 조상래 | - |
dc.contributor.author | 조양제 | - |
dc.date.accessioned | 2017-09-29T06:33:47Z | - |
dc.date.available | 2017-09-29T06:33:47Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1229-4101 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/149941 | - |
dc.description.abstract | Background: Recently, the mitochondrial proapoptotic protein, apoptosis-inducing factor (AIF), and its nuclear translocation have been reported in caspase-independent neuronal apoptosis. However, it is not elucidated whether oxidative signaling is involved in the nuclear translocation of AIF and subsequent caspase-independent apoptotic cell death. We investigated whether oxidative signaling induces nuclear translocation of AIF and subsequent caspase- independent apoptosis-associated DNA fragmentation after permanent focal cerebral ischemia (FCI). Methods: Adult male ICR mice were subjected to permanent FCI by intraluminal suture blockade of middle cerebral artery. Immunohistochemistry and Western blot analysis were performed. Large-scale DNA fragmentation was evaluated by pulse field gel electrophoresis and apoptotic cell death was quantified. Manganese tetrakis (4-benzoic acid) porphyrin (MnTBAP), which mimics mitochondrial superoxide dismutase, was used to determinewhether the production of reactive oxygen species is required for the induction in AIF translocation. Results: Western blot analysis showed that the nuclear translocation of AIF occurred as early as 2 hours after permanent FCI. Immunostaining for AIF also confirmed early nuclear translocation of AIF after permanent FCI. Large-scale DNA fragmentation was detected 8 hours after permanent FCI. MnTBAP-treatment attenuated AIF translocation and blocked large-scale DNA fragmentation. Caspase-3 activity was similarly inhibited between the pan-caspase inhibitor- and MnTBAP-treated mice, but the amount of apoptosis-associated DNA fragmentation in the MnTBAP-treated mice was less than in the pan-caspase inhibitor-treated mice (P<0.001). In addition, infarction volume was also decreased by MnTBAP. Conclusions: These results suggest that reactive oxygen species induce the caspase-independent nuclear translocation of AIF and subsequent apoptosis-associated DNA fragmentation after permanent FCI. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한뇌졸중학회 | - |
dc.relation.isPartOf | Korean Journal of Stroke (대한뇌졸중학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Ischemic brain injury | - |
dc.subject.MESH | Oxidative stress | - |
dc.subject.MESH | Apoptosis-inducing factor | - |
dc.title | 영구적 국소 대뇌 허혈 생쥐 모델에서 활성 산소종에 의한 세포고사 유도인자의 핵내 이동과 세포고사 | - |
dc.title.alternative | Nuclear Translocation of Apoptosis Inducing Factor and Subsequent DNA Fragmentation by Reactive Oxygen Species After Permanent Focal Cerebral Ischemia in Mice | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurology (신경과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Dept. of Neurology (신경과학교실) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.department | Dept. of Neurology (신경과학교실) | - |
dc.contributor.googleauthor | 조양제 | - |
dc.contributor.googleauthor | 이용현 | - |
dc.contributor.googleauthor | 조경주 | - |
dc.contributor.googleauthor | 이두재 | - |
dc.contributor.googleauthor | 김현우 | - |
dc.contributor.googleauthor | 김현정 | - |
dc.contributor.googleauthor | 이병인 | - |
dc.contributor.googleauthor | 조상래 | - |
dc.contributor.googleauthor | 김경환 | - |
dc.identifier.doi | OAK-2005-06528 | - |
dc.contributor.localId | A00310 | - |
dc.contributor.localId | A01125 | - |
dc.contributor.localId | A02797 | - |
dc.contributor.localId | A03824 | - |
dc.contributor.localId | A03851 | - |
dc.relation.journalcode | J02121 | - |
dc.relation.journalsince | 1999~2011 | - |
dc.relation.journalafter | 2013~ Journal of Stroke | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Ischemic brain injury | - |
dc.subject.keyword | Oxidative stress | - |
dc.subject.keyword | Apoptosis-inducing factor | - |
dc.contributor.alternativeName | Kim, Gyung Whan | - |
dc.contributor.alternativeName | Kim, Hyun Woo | - |
dc.contributor.alternativeName | Lee, Byung In | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.alternativeName | Cho, Yang Je | - |
dc.citation.volume | 7 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 214 | - |
dc.citation.endPage | 223 | - |
dc.identifier.bibliographicCitation | Korean Journal of Stroke (대한뇌졸중학회지), Vol.7(2) : 214-223, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 42030 | - |
dc.type.rims | ART | - |
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