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체중부하 변화에 따른 골성장판내 세포동력학적 변화

Other Titles
 Altered Cellular Kinetics in the Growth Plate according to Alterations in the Weight Bearing 
 강호정  ;  공선영  ;  박건보  ;  주선영  ;  양익환  ;  박희완  ;  김현우 
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.8(1) : 50-58, 2005 
Journal Title
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) 
Issue Date
Cellular kinetics ; Growth plate ; Changes in weight bearing
Cellular kinetics ; Growth plate ; Changes in weight bearing
Purpose: To examine the effects of change in the weight bearing on the growth plate metabolism, a simulated animal model of weightlessness was introduced and the chondrocytes’ cellular kinetics were evaluated. Materials and Methods: Unloading condition on the hind-limb of Sprague-Dawley rats was created by fixing a tail and lifting the hind-limb. Six rats aged 6 weeks old were assigned to each group of unloading, reloading, and control groups of unloading or reloading. Unloading was maintained for three weeks, and then reloading was applied for another one week thereafter. Histomorphometry for the assessment of vertical length of the growth plate, 5-bromo-2′-deoxyuridin (BrdU) immunohistochemistry for cellular kinetics, and biotin nick end labeling TUNEL assay for chondrocytes in the growth plate were performed. Results: The vertical length of the growth plate and the proliferative potential of chondrocytes were decreased in the unloading group than those of control groups. Inter-group differences were more significant in the proliferative and hypertrophic zones. Reloading increased the length of growth plate and proliferative potential of chondrocytes as evidenced by increase of the ratio of positive BrdU stained cells. However, apoptotic changes in the growth plate were not affected by the alterations of weight bearing. Conclusion: Alterations in the weight bearing induced changes in the chondrocytic proliferative potential of the growth plate and have no effects on the apoptosis occurred. This may suggest that deprived weight bearing due to various clinical situations hamper normal longitudinal bone growth, and further studies regarding thebfactors for reversibility of chontrocytic proliferation upon variable mechanical stresses are needed.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kang, Ho Jung(강호정) ORCID logo https://orcid.org/0000-0003-0273-1264
Kong, Sun Young(공선영)
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Park, Kun Bo(박건보) ORCID logo https://orcid.org/0000-0002-8839-4870
Park, Hui Wan(박희완)
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