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IL-1β promotes the ciliogenesis of human middle ear epithelial cells: possible linkage with the expression of mucin gene 8

Authors
 Jae Young Choi  ;  Jin-Young Kim  ;  Chang-Woo Kim  ;  Jung Sang Ho  ;  Kyung-Dong Lee  ;  Jong-Bum Yoo  ;  Young Eun Ahn  ;  Joo-Heon Yoon 
Citation
 ACTA OTO-LARYNGOLOGICA, Vol.125(3) : 260-265, 2005 
Journal Title
ACTA OTO-LARYNGOLOGICA
ISSN
 0001-6489 
Issue Date
2005
MeSH
Cilia ; inflammation ; otitis media
Keywords
Cilia ; inflammation ; otitis media
Abstract
CONCLUSIONS: These findings suggest that IL-1beta, increases ciliogenesis in NHMEE cells and that MUC8 protein may play a role in this process.
OBJECTIVES: The morphology of the middle ear epithelium changes under various pathologic conditions. We compared the density of ciliated cells and the level of mucin gene 8 (MUC8) mRNA in the middle ear epithelial cells of mucoid otitis media (MOM) patients with those of normal controls. We also investigated the effect of IL-1beta on the ciliogenesis and expression of MUC8 mRNA in cultured normal human middle ear epithelial (NHMEE) cells. In addition, we localized the MUC8 protein in cultured NHMEE cells.
MATERIAL AND METHODS: The surface morphology of NHMEE cells was examined using scanning electron microscopy. Reverse transcriptase polymerase chain reaction was performed to determine the level of MUC8 mRNA expression. After synthesis of MUC8 polyclonal antibody, we performed immunohistochemical staining.
RESULTS: The density of ciliated cells was increased in the middle ear epithelium of both MOM patients and IL-1beta-treated cultures. Similarly, the expression of MUC8 was increased in both cases. MUC8 antibody was primarily stained on the ciliated cells of NHMEE cells.
Full Text
http://informahealthcare.com/doi/abs/10.1080/00016480410022985
DOI
10.1080/00016480410022985
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Ahn, Young Eun(안영은)
Yoo, Jong Bum(유종범)
Yoon, Joo Heon(윤주헌)
Jung, Sang Ho(정상호)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147532
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