Cited 8 times in
Production of bone morphogenetic protein-7 using pET expression system
DC Field | Value | Language |
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dc.contributor.author | 박종철 | - |
dc.contributor.author | 백현숙 | - |
dc.contributor.author | 이동희 | - |
dc.contributor.author | 이미희 | - |
dc.date.accessioned | 2017-05-04T07:34:54Z | - |
dc.date.available | 2017-05-04T07:34:54Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1567-1739 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147496 | - |
dc.description.abstract | The polypeptide representing the mature part of human bone morphogenetic protein-7 (BMP-7) was efficiently expressed in E. coli BL21 (DE3). The rhBMP-7 is a disulfide-bonded homodimeric protein with an apparent MW of 29,000 as shown by the SDS-PAGE and gel chromatography. rhBMP-7 stimulates ALP specific activity in a dose-dependent manner. In a kinetic experiment, ALP activity in both the rhBMP-7 (100 ng/ml)-treated and control cultures increases gradually, and activity in the rhBMP-7-treated culture is consistently higher throughout the culture period. We also quantitated the specific osteoblastic marker osteocalcin in rhBMP-7-treated culture medium. When serum-starved MC3T3-E1 cells are treated with medium containing various concentrations of rhBMP-7, the production of osteocalcin is increased about 7.2-fold in a dose-dependent manner up to 320 ng rhBMP-7/ml. In summary, we established an E. coli expression system for a high level of rhBMP-7 production and inductive effects of osteogenic differentiation markers by rhBMP-7 in MC3T3-E1 cells were evaluated. rhBMP-7 is strongly mitogenic for MC3T3-E1 cells, showing dose-dependent induction of ALP activity and osteocalcin production. Thus our expression system provides a convenient source of rhBMPs for in vitro or in vivo study. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | CURRENT APPLIED PHYSICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Bone morphogenetic protein | - |
dc.subject.MESH | E. coli | - |
dc.subject.MESH | Inclusion body | - |
dc.subject.MESH | n-Cethyltrimethylammonium chloride | - |
dc.subject.MESH | Refolding | - |
dc.subject.MESH | Osteoblast | - |
dc.title | Production of bone morphogenetic protein-7 using pET expression system | - |
dc.type | Article | - |
dc.publisher.location | Netherlands | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | Others | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학교실) | - |
dc.contributor.department | Severance Hospital (세브란스병원) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학교실) | - |
dc.contributor.googleauthor | Lee Dong Hee | - |
dc.contributor.googleauthor | Baek Hyun Sook | - |
dc.contributor.googleauthor | Lee Mi Hee | - |
dc.contributor.googleauthor | Park Jong-Chul | - |
dc.identifier.doi | 10.1016/j.cap.2005.01.003 | - |
dc.contributor.localId | A01662 | - |
dc.contributor.localId | A01843 | - |
dc.contributor.localId | A02744 | - |
dc.contributor.localId | A02777 | - |
dc.relation.journalcode | J00659 | - |
dc.identifier.eissn | 1878-1675 | - |
dc.identifier.pmid | 10.1016/j.cap.2005.01.003 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1567173905000076 | - |
dc.subject.keyword | Bone morphogenetic protein | - |
dc.subject.keyword | E. coli | - |
dc.subject.keyword | Inclusion body | - |
dc.subject.keyword | n-Cethyltrimethylammonium chloride | - |
dc.subject.keyword | Refolding | - |
dc.subject.keyword | Osteoblast | - |
dc.contributor.alternativeName | Park, Jong Chul | - |
dc.contributor.alternativeName | Baek, Hyun Sook | - |
dc.contributor.alternativeName | Lee, Dong Hee | - |
dc.contributor.alternativeName | Lee, Mi Hee | - |
dc.citation.volume | 5 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 422 | - |
dc.citation.endPage | 425 | - |
dc.identifier.bibliographicCitation | CURRENT APPLIED PHYSICS, Vol.5(5) : 422-425, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 40275 | - |
dc.type.rims | ART | - |
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