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Isolation of Endothelial Progenitor Cells from Cord Blood and Induction of Differentiation by Ex Vivo Expansion

 Jeong-Won Shin  ;  Dong-Wha Lee  ;  Moon-Jung Kim  ;  Kyung-Soon Song  ;  Han-Soo Kim  ;  Hyun-Ok Kim 
 YONSEI MEDICAL JOURNAL, Vol.46(2) : 260-267, 2005 
Journal Title
Issue Date
Antigens, CD34/analysis ; Cell Differentiation/drug effects ; Cell Division/drug effects ; Cell Separation* ; Cells, Cultured ; Cytokines/pharmacology ; Endothelial Cells*/immunology ; Fetal Blood/cytology* ; Fetus ; Flow Cytometry ; Humans ; Stem Cells*/immunology* ; Thrombopoietin/pharmacology
Endothellial progenitor cells ; neovascularization ; thrombopoietin
Endothelial progenitor cells (EPCs) have been reported to possess the capacity to colonize vascular grafts and hold promise for therapeutic neovascularization. However, limited quantities of EPCs have been the major factor impeding effective research on vasculoangiogenesis. In this study, cytokine and culture conditions necessary for the provision of large quantities of endothelial cells (ECs) were investigated. Cord blood was collected from 18 normal full-term deliveries and CD34+ cells were isolated by MACS system (Miltenyi Biotech, Bergish-Gladbach, Germany). To evaluate the effect of cytokines, CD34+ cells were cultured with various cytokine combinations, such as stem cell factor (SCF), flt3-ligand (FL), and thrombopoietin (TPO) with vascular endothelial growth factor (VEGF), interleukin-1β, fibroblast growth factor-basic (FGF-b) as basic cytokines. The quantities of non-adherent and adherent cells were the greatest with SCF, FL and TPO. The addition of TPO to all other cytokines significantly increased the number of non-adherent and adherent cells (p < 0.05, Wilcoxon rank sum test). After four weeks of culture, adherent cells expressed endothelial specific markers such as KDR, CD31 and CD62E. Typical morphology of ECs was observed during culture, such as cord-like structure and cobblestone appearance, suggesting that the adherent cells were consistent with ECs. In this study, the experimental conditions that optimize the production of ECs for therapeutic neovascularization were described. And it was possibly suggested that TPO plays a major role in differentiation from EPCs to ECs.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Han Soo(김한수)
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Song, Kyung Soon(송경순)
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