Cited 11 times in
In vitro bioassay of endotoxin using fluorescein as a pH indicator in a macrophage cell culture system
DC Field | Value | Language |
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dc.contributor.author | 박종철 | - |
dc.contributor.author | 성학준 | - |
dc.contributor.author | 이동희 | - |
dc.contributor.author | 이민섭 | - |
dc.contributor.author | 한동욱 | - |
dc.date.accessioned | 2017-05-04T07:29:42Z | - |
dc.date.available | 2017-05-04T07:29:42Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147369 | - |
dc.description.abstract | Based on the biological activity of endotoxin, we propose a possible new method for detecting endotoxin using a pH-indication system of macrophage culture media. After RAW 264.7 macrophage cells were treated with lipopolysaccharide (LPS), the addition of fluorescein to the LPS-treated media reproductively reduced its absorption and emission spectra (it was a dose-dependent reduction). The advantages of this LPS-detection method were compared with the Limulus Amebocyte Lysate (LAL) test by using purified bacterial LPS (Salmonella minnessota, Escherichia coli, and Pseudomonas aeruginosa). Additionally, the absorption and fluorescence intensity of fluorescein, following treatment of RAW 264.7 cells with a high concentration of Staphylococcus aureus (Gram-positive, lysed bacteria), could not generally be detected by the LAL test, but they were found to be reduced, in a dose-response relationship, with this new system. The macrophage culture system-method might be a good supplement to the LAL assay for detection of LPS, Gram-negative and Gram-positive bacteria. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Biological Assay/methods* | - |
dc.subject.MESH | Biological Assay/standards | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Contrast Media* | - |
dc.subject.MESH | Culture Media/chemistry | - |
dc.subject.MESH | Endotoxins/analysis* | - |
dc.subject.MESH | Fluorescein* | - |
dc.subject.MESH | Hydrogen-Ion Concentration | - |
dc.subject.MESH | Limulus Test | - |
dc.subject.MESH | Lipopolysaccharides/analysis | - |
dc.subject.MESH | Macrophages/chemistry* | - |
dc.subject.MESH | Mice | - |
dc.title | In vitro bioassay of endotoxin using fluorescein as a pH indicator in a macrophage cell culture system | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학교실) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학교실) | - |
dc.contributor.googleauthor | Dong Hee Lee | - |
dc.contributor.googleauthor | Hak-Joon Sung | - |
dc.contributor.googleauthor | Dong-Wook Han | - |
dc.contributor.googleauthor | Min-Sub Lee | - |
dc.contributor.googleauthor | Gyu Ha Ryu | - |
dc.contributor.googleauthor | Maki Aihara | - |
dc.contributor.googleauthor | Kosuke Takatori | - |
dc.contributor.googleauthor | Jong-Chul Park | - |
dc.identifier.doi | 10.3349/ymj.2005.46.2.268 | - |
dc.contributor.localId | A01662 | - |
dc.contributor.localId | A01958 | - |
dc.contributor.localId | A02744 | - |
dc.contributor.localId | A02782 | - |
dc.contributor.localId | A04275 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 15861501 | - |
dc.subject.keyword | Lipopolysaccharide | - |
dc.subject.keyword | macrophage | - |
dc.subject.keyword | fluorescein | - |
dc.subject.keyword | Limulus Amebocyte Lysate test | - |
dc.subject.keyword | Staphylococcus aureus | - |
dc.subject.keyword | pH | - |
dc.contributor.alternativeName | Park, Jong Chul | - |
dc.contributor.alternativeName | Sung, Hak-Joon | - |
dc.contributor.alternativeName | Lee, Dong Hee | - |
dc.contributor.alternativeName | Lee, Min Sub | - |
dc.contributor.alternativeName | Han, Dong Wook | - |
dc.citation.volume | 46 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 268 | - |
dc.citation.endPage | 274 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.46(2) : 268-274, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 48541 | - |
dc.type.rims | ART | - |
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