Cited 0 times in
E1A/E1B 이중변이형 종양 선택적 살상 아데노바이러스의 개선된 암세포 살상 효과 및 항종양 효과
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김재성 | - |
dc.contributor.author | 김주항 | - |
dc.contributor.author | 김평환 | - |
dc.contributor.author | 손주혁 | - |
dc.contributor.author | 윤채옥 | - |
dc.contributor.author | 최경주 | - |
dc.date.accessioned | 2017-05-04T07:27:36Z | - |
dc.date.available | 2017-05-04T07:27:36Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1598-2467 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147319 | - |
dc.description.abstract | Gene-modified replication-competent adenoviruses (Ads) are emerging as a promising new modality for the treatment of cancer. We have previously shown that E1B 19kDa and E1B 55kDa gene deleted Ad (Ad-△E1B19/55) exhibits improved tumor-specific replication and cell lysis, leading to potent anti-tumor effect. As an additional effort to increase cancer cell-selectivity of replicating adenovirus, we have first generated eleven E1A-mutant Ads (Ad-mt#1~#11) with deletion or substitution in retinoblastoma (Rb) binding sites of E1A. Of these viruses, Ad-mt#7, demonstrated Significantly improved cytopathic effect (CPE) and viral replication in a cancer cell-specific manner. To further increase the cancer cell-specific killing effect of Ad-mt#7, both E1B 19kDa and E1B 55kDa genes were deleted, resulting in an Ad-△E1Bmt7. As assessed using CPE assay, MTT assay, and immunoblot analysis for Ad fiber expression, Ad-△E1Bmt7 exerted markeldly enhanced cancer cell-specific killing effect as well as viral replication in comparison to either Ad-mt#7 or Ad-△E1B19/55. Furthermore, the growth of established human cervical carcinoma in nude mice was significantly suppressed by intratumoral injection of Ad-△E1Bmt7. In summary, we have developed an oncolytic adenovirus with significantly improved therapeutic profiles for cancer treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 113~124 | - |
dc.language | English, Korean | - |
dc.publisher | Seo Heung Pub. Co. | - |
dc.relation.isPartOf | Journal of Bacteriology and Virology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | cancer gene therapy | - |
dc.subject.MESH | conditionally-replicating adenovirus | - |
dc.subject.MESH | apoptosis | - |
dc.title | E1A/E1B 이중변이형 종양 선택적 살상 아데노바이러스의 개선된 암세포 살상 효과 및 항종양 효과 | - |
dc.title.alternative | Enhanced Oncolytic Effect and Anti-Tumor Effect of Replication Competent Adenovirus with Double Mutation in E1A & E1B Regions | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | Research Institutes (연구소) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Institute for Cancer Research (암연구소) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | 김재성 | - |
dc.contributor.googleauthor | 최경주 | - |
dc.contributor.googleauthor | 김평환 | - |
dc.contributor.googleauthor | 김주항 | - |
dc.contributor.googleauthor | 손주혁 | - |
dc.contributor.googleauthor | 윤채옥 | - |
dc.identifier.doi | OAK-2005-02461 | - |
dc.contributor.localId | A00860 | - |
dc.contributor.localId | A00945 | - |
dc.contributor.localId | A01088 | - |
dc.contributor.localId | A01995 | - |
dc.contributor.localId | A02614 | - |
dc.contributor.localId | A04034 | - |
dc.relation.journalcode | J01255 | - |
dc.identifier.eissn | 2093-0429 | - |
dc.subject.keyword | cancer gene therapy | - |
dc.subject.keyword | conditionally-replicating adenovirus | - |
dc.subject.keyword | apoptosis | - |
dc.contributor.alternativeName | Kim, Jaesung | - |
dc.contributor.alternativeName | Kim, Joo Hang | - |
dc.contributor.alternativeName | Kim, Pyung Hwan | - |
dc.contributor.alternativeName | Sohn, Joo Hyuk | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.alternativeName | Choi, Kyung Ju | - |
dc.citation.volume | 35 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 113 | - |
dc.citation.endPage | 124 | - |
dc.identifier.bibliographicCitation | Journal of Bacteriology and Virology, Vol.35(2) : 113-124, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 48491 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.