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E1A/E1B 이중변이형 종양 선택적 살상 아데노바이러스의 개선된 암세포 살상 효과 및 항종양 효과

DC FieldValueLanguage
dc.contributor.author김재성-
dc.contributor.author김주항-
dc.contributor.author김평환-
dc.contributor.author손주혁-
dc.contributor.author윤채옥-
dc.contributor.author최경주-
dc.date.accessioned2017-05-04T07:27:36Z-
dc.date.available2017-05-04T07:27:36Z-
dc.date.issued2005-
dc.identifier.issn1598-2467-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/147319-
dc.description.abstractGene-modified replication-competent adenoviruses (Ads) are emerging as a promising new modality for the treatment of cancer. We have previously shown that E1B 19kDa and E1B 55kDa gene deleted Ad (Ad-△E1B19/55) exhibits improved tumor-specific replication and cell lysis, leading to potent anti-tumor effect. As an additional effort to increase cancer cell-selectivity of replicating adenovirus, we have first generated eleven E1A-mutant Ads (Ad-mt#1~#11) with deletion or substitution in retinoblastoma (Rb) binding sites of E1A. Of these viruses, Ad-mt#7, demonstrated Significantly improved cytopathic effect (CPE) and viral replication in a cancer cell-specific manner. To further increase the cancer cell-specific killing effect of Ad-mt#7, both E1B 19kDa and E1B 55kDa genes were deleted, resulting in an Ad-△E1Bmt7. As assessed using CPE assay, MTT assay, and immunoblot analysis for Ad fiber expression, Ad-△E1Bmt7 exerted markeldly enhanced cancer cell-specific killing effect as well as viral replication in comparison to either Ad-mt#7 or Ad-△E1B19/55. Furthermore, the growth of established human cervical carcinoma in nude mice was significantly suppressed by intratumoral injection of Ad-△E1Bmt7. In summary, we have developed an oncolytic adenovirus with significantly improved therapeutic profiles for cancer treatment.-
dc.description.statementOfResponsibilityopen-
dc.format.extent113~124-
dc.languageEnglish, Korean-
dc.publisherSeo Heung Pub. Co.-
dc.relation.isPartOfJournal of Bacteriology and Virology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHcancer gene therapy-
dc.subject.MESHconditionally-replicating adenovirus-
dc.subject.MESHapoptosis-
dc.titleE1A/E1B 이중변이형 종양 선택적 살상 아데노바이러스의 개선된 암세포 살상 효과 및 항종양 효과-
dc.title.alternativeEnhanced Oncolytic Effect and Anti-Tumor Effect of Replication Competent Adenovirus with Double Mutation in E1A & E1B Regions-
dc.typeArticle-
dc.publisher.locationKorea (South)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeResearch Institutes (연구소)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.departmentInstitute for Cancer Research (암연구소)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthor김재성-
dc.contributor.googleauthor최경주-
dc.contributor.googleauthor김평환-
dc.contributor.googleauthor김주항-
dc.contributor.googleauthor손주혁-
dc.contributor.googleauthor윤채옥-
dc.identifier.doiOAK-2005-02461-
dc.contributor.localIdA00860-
dc.contributor.localIdA00945-
dc.contributor.localIdA01088-
dc.contributor.localIdA01995-
dc.contributor.localIdA02614-
dc.contributor.localIdA04034-
dc.relation.journalcodeJ01255-
dc.identifier.eissn2093-0429-
dc.subject.keywordcancer gene therapy-
dc.subject.keywordconditionally-replicating adenovirus-
dc.subject.keywordapoptosis-
dc.contributor.alternativeNameKim, Jaesung-
dc.contributor.alternativeNameKim, Joo Hang-
dc.contributor.alternativeNameKim, Pyung Hwan-
dc.contributor.alternativeNameSohn, Joo Hyuk-
dc.contributor.alternativeNameYun, Chae Ok-
dc.contributor.alternativeNameChoi, Kyung Ju-
dc.citation.volume35-
dc.citation.number2-
dc.citation.startPage113-
dc.citation.endPage124-
dc.identifier.bibliographicCitationJournal of Bacteriology and Virology, Vol.35(2) : 113-124, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Cancer Research (암연구소) > 1. Journal Papers

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