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Reduced expression of granule proteins during extended survival of eosinophils in splenocyte culture with GM-CSF.

Authors
 Seul Hye Ryu  ;  Hye Young Na  ;  Moah Sohn  ;  Sun Murray Han  ;  Wanho Choi  ;  Hyunju In  ;  Sookyung Hong  ;  Hyejin Jeon  ;  Jun-Young Seo  ;  Jongcheol Ahn  ;  Chae Gyu Park 
Citation
 IMMUNOLOGY LETTERS, Vol.173 : 7-20, 2016 
Journal Title
 IMMUNOLOGY LETTERS 
ISSN
 0165-2478 
Issue Date
2016
MeSH
Animals ; Cell Differentiation ; Cell Movement ; Cell Survival ; Cells, Cultured ; Chemokine CCL11/immunology ; Cytoplasmic Granules/metabolism ; Eosinophil Major Basic Protein/genetics ; Eosinophil Major Basic Protein/metabolism ; Eosinophil Peroxidase/genetics ; Eosinophil Peroxidase/metabolism ; Eosinophils/immunology* ; Gene Expression Regulation ; Granulocyte-Macrophage Colony-Stimulating Factor/metabolism* ; Hematopoiesis ; Mice ; Mice, Inbred C57BL ; Receptors, CCR3/metabolism ; Sialic Acid Binding Ig-like Lectin 1/metabolism ; Spleen/immunology* ; Spleen/pathology
Keywords
Eosinophils ; GM-CSF ; Gene expression ; Granule protein ; Mouse ; Spleen
Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multifaceted hematopoietic cytokine and the culture of mouse bone marrow with GM-CSF produces a variety of myeloid cells including granulocytes, macrophages, and dendritic cells. In the present study, we cultured mouse splenocytes with GM-CSF and examined the changes in hematopoietic cell populations over a week. Most of the splenic hematopoietic cells disappeared significantly from culture within 6days with or without the presence of GM-CSF. Among the splenic granulocyte populations, only eosinophils fully survived throughout the culture with GM-CSF for more than a week. During 10days of culture with GM-CSF, splenic eosinophils maintained their morphology as well as most of their surface molecules at high levels, including CCR3 and Siglec F. Meanwhile, the expression of mRNAs encoding major basic protein-1 (MBP-1) and eosinophil peroxidase (EPO), two major eosinophil-derived granule proteins, was diminished significantly from the cultured eosinophils. EPO assays also revealed that eosinophils in culture for more than 5days retained 30% or less EPO activity compared to those in uncultured splenocytes. In contrast, culture of splenocytes with GM-CSF did not change the capacity of eosinophils to migrate in response to eotaxin-1. Our results indicate that mouse splenic eosinophils are effectively cultured for lengthy periods while their expression of eosinophil-derived granule proteins is specifically suppressed. The relevance of these findings to eosinophilic inflammatory response is discussed.
Full Text
http://www.sciencedirect.com/science/article/pii/S0165247816300281
DOI
10.1016/j.imlet.2016.03.003
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Na, Hye Young(나혜영) ORCID logo https://orcid.org/0000-0002-2886-9926
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
Seo, Jun Young(서준영) ORCID logo https://orcid.org/0000-0003-4004-2013
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146996
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