Cited 11 times in
Reduced expression of granule proteins during extended survival of eosinophils in splenocyte culture with GM-CSF.
DC Field | Value | Language |
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dc.contributor.author | 나혜영 | - |
dc.contributor.author | 박채규 | - |
dc.contributor.author | 서준영 | - |
dc.date.accessioned | 2017-02-27T07:48:56Z | - |
dc.date.available | 2017-02-27T07:48:56Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/146996 | - |
dc.description.abstract | Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multifaceted hematopoietic cytokine and the culture of mouse bone marrow with GM-CSF produces a variety of myeloid cells including granulocytes, macrophages, and dendritic cells. In the present study, we cultured mouse splenocytes with GM-CSF and examined the changes in hematopoietic cell populations over a week. Most of the splenic hematopoietic cells disappeared significantly from culture within 6days with or without the presence of GM-CSF. Among the splenic granulocyte populations, only eosinophils fully survived throughout the culture with GM-CSF for more than a week. During 10days of culture with GM-CSF, splenic eosinophils maintained their morphology as well as most of their surface molecules at high levels, including CCR3 and Siglec F. Meanwhile, the expression of mRNAs encoding major basic protein-1 (MBP-1) and eosinophil peroxidase (EPO), two major eosinophil-derived granule proteins, was diminished significantly from the cultured eosinophils. EPO assays also revealed that eosinophils in culture for more than 5days retained 30% or less EPO activity compared to those in uncultured splenocytes. In contrast, culture of splenocytes with GM-CSF did not change the capacity of eosinophils to migrate in response to eotaxin-1. Our results indicate that mouse splenic eosinophils are effectively cultured for lengthy periods while their expression of eosinophil-derived granule proteins is specifically suppressed. The relevance of these findings to eosinophilic inflammatory response is discussed. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.format.extent | 7~20 | - |
dc.language | English | - |
dc.publisher | Elsevier/North-Holland Biomedical Press | - |
dc.relation.isPartOf | IMMUNOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Movement | - |
dc.subject.MESH | Cell Survival | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chemokine CCL11/immunology | - |
dc.subject.MESH | Cytoplasmic Granules/metabolism | - |
dc.subject.MESH | Eosinophil Major Basic Protein/genetics | - |
dc.subject.MESH | Eosinophil Major Basic Protein/metabolism | - |
dc.subject.MESH | Eosinophil Peroxidase/genetics | - |
dc.subject.MESH | Eosinophil Peroxidase/metabolism | - |
dc.subject.MESH | Eosinophils/immunology* | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor/metabolism* | - |
dc.subject.MESH | Hematopoiesis | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Receptors, CCR3/metabolism | - |
dc.subject.MESH | Sialic Acid Binding Ig-like Lectin 1/metabolism | - |
dc.subject.MESH | Spleen/immunology* | - |
dc.subject.MESH | Spleen/pathology | - |
dc.title | Reduced expression of granule proteins during extended survival of eosinophils in splenocyte culture with GM-CSF. | - |
dc.type | Article | - |
dc.publisher.location | Netherlands | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Life Science | - |
dc.contributor.googleauthor | Seul Hye Ryu | - |
dc.contributor.googleauthor | Hye Young Na | - |
dc.contributor.googleauthor | Moah Sohn | - |
dc.contributor.googleauthor | Sun Murray Han | - |
dc.contributor.googleauthor | Wanho Choi | - |
dc.contributor.googleauthor | Hyunju In | - |
dc.contributor.googleauthor | Sookyung Hong | - |
dc.contributor.googleauthor | Hyejin Jeon | - |
dc.contributor.googleauthor | Jun-Young Seo | - |
dc.contributor.googleauthor | Jongcheol Ahn | - |
dc.contributor.googleauthor | Chae Gyu Park | - |
dc.identifier.doi | 10.1016/j.imlet.2016.03.003 | - |
dc.contributor.localId | A04556 | - |
dc.contributor.localId | A01718 | - |
dc.contributor.localId | A01911 | - |
dc.relation.journalcode | J01038 | - |
dc.identifier.eissn | 1879-0542 | - |
dc.identifier.pmid | 26969350 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0165247816300281 | - |
dc.subject.keyword | Eosinophils | - |
dc.subject.keyword | GM-CSF | - |
dc.subject.keyword | Gene expression | - |
dc.subject.keyword | Granule protein | - |
dc.subject.keyword | Mouse | - |
dc.subject.keyword | Spleen | - |
dc.contributor.alternativeName | Na, Hye Young | - |
dc.contributor.alternativeName | Park, Chae Gyu | - |
dc.contributor.alternativeName | Seo, Jun Young | - |
dc.contributor.affiliatedAuthor | Na, Hye Young | - |
dc.contributor.affiliatedAuthor | Park, Chae Gyu | - |
dc.contributor.affiliatedAuthor | Seo, Jun Young | - |
dc.citation.volume | 173 | - |
dc.citation.startPage | 7 | - |
dc.citation.endPage | 20 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY LETTERS, Vol.173 : 7-20, 2016 | - |
dc.date.modified | 2017-02-24 | - |
dc.identifier.rimsid | 47029 | - |
dc.type.rims | ART | - |
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