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Reduced expression of granule proteins during extended survival of eosinophils in splenocyte culture with GM-CSF.

DC FieldValueLanguage
dc.contributor.author나혜영-
dc.contributor.author박채규-
dc.contributor.author서준영-
dc.date.accessioned2017-02-27T07:48:56Z-
dc.date.available2017-02-27T07:48:56Z-
dc.date.issued2016-
dc.identifier.issn0165-2478-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146996-
dc.description.abstractGranulocyte-macrophage colony-stimulating factor (GM-CSF) is a multifaceted hematopoietic cytokine and the culture of mouse bone marrow with GM-CSF produces a variety of myeloid cells including granulocytes, macrophages, and dendritic cells. In the present study, we cultured mouse splenocytes with GM-CSF and examined the changes in hematopoietic cell populations over a week. Most of the splenic hematopoietic cells disappeared significantly from culture within 6days with or without the presence of GM-CSF. Among the splenic granulocyte populations, only eosinophils fully survived throughout the culture with GM-CSF for more than a week. During 10days of culture with GM-CSF, splenic eosinophils maintained their morphology as well as most of their surface molecules at high levels, including CCR3 and Siglec F. Meanwhile, the expression of mRNAs encoding major basic protein-1 (MBP-1) and eosinophil peroxidase (EPO), two major eosinophil-derived granule proteins, was diminished significantly from the cultured eosinophils. EPO assays also revealed that eosinophils in culture for more than 5days retained 30% or less EPO activity compared to those in uncultured splenocytes. In contrast, culture of splenocytes with GM-CSF did not change the capacity of eosinophils to migrate in response to eotaxin-1. Our results indicate that mouse splenic eosinophils are effectively cultured for lengthy periods while their expression of eosinophil-derived granule proteins is specifically suppressed. The relevance of these findings to eosinophilic inflammatory response is discussed.-
dc.description.statementOfResponsibilityrestriction-
dc.format.extent7~20-
dc.languageEnglish-
dc.publisherElsevier/North-Holland Biomedical Press-
dc.relation.isPartOfIMMUNOLOGY LETTERS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Movement-
dc.subject.MESHCell Survival-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChemokine CCL11/immunology-
dc.subject.MESHCytoplasmic Granules/metabolism-
dc.subject.MESHEosinophil Major Basic Protein/genetics-
dc.subject.MESHEosinophil Major Basic Protein/metabolism-
dc.subject.MESHEosinophil Peroxidase/genetics-
dc.subject.MESHEosinophil Peroxidase/metabolism-
dc.subject.MESHEosinophils/immunology*-
dc.subject.MESHGene Expression Regulation-
dc.subject.MESHGranulocyte-Macrophage Colony-Stimulating Factor/metabolism*-
dc.subject.MESHHematopoiesis-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHReceptors, CCR3/metabolism-
dc.subject.MESHSialic Acid Binding Ig-like Lectin 1/metabolism-
dc.subject.MESHSpleen/immunology*-
dc.subject.MESHSpleen/pathology-
dc.titleReduced expression of granule proteins during extended survival of eosinophils in splenocyte culture with GM-CSF.-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Life Science-
dc.contributor.googleauthorSeul Hye Ryu-
dc.contributor.googleauthorHye Young Na-
dc.contributor.googleauthorMoah Sohn-
dc.contributor.googleauthorSun Murray Han-
dc.contributor.googleauthorWanho Choi-
dc.contributor.googleauthorHyunju In-
dc.contributor.googleauthorSookyung Hong-
dc.contributor.googleauthorHyejin Jeon-
dc.contributor.googleauthorJun-Young Seo-
dc.contributor.googleauthorJongcheol Ahn-
dc.contributor.googleauthorChae Gyu Park-
dc.identifier.doi10.1016/j.imlet.2016.03.003-
dc.contributor.localIdA04556-
dc.contributor.localIdA01718-
dc.contributor.localIdA01911-
dc.relation.journalcodeJ01038-
dc.identifier.eissn1879-0542-
dc.identifier.pmid26969350-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0165247816300281-
dc.subject.keywordEosinophils-
dc.subject.keywordGM-CSF-
dc.subject.keywordGene expression-
dc.subject.keywordGranule protein-
dc.subject.keywordMouse-
dc.subject.keywordSpleen-
dc.contributor.alternativeNameNa, Hye Young-
dc.contributor.alternativeNamePark, Chae Gyu-
dc.contributor.alternativeNameSeo, Jun Young-
dc.contributor.affiliatedAuthorNa, Hye Young-
dc.contributor.affiliatedAuthorPark, Chae Gyu-
dc.contributor.affiliatedAuthorSeo, Jun Young-
dc.citation.volume173-
dc.citation.startPage7-
dc.citation.endPage20-
dc.identifier.bibliographicCitationIMMUNOLOGY LETTERS, Vol.173 : 7-20, 2016-
dc.date.modified2017-02-24-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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