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Role of Thalidomide on the Expression of OX40, 4-1BB, and GITR in T Cell Subsets

Authors
 B.S. Kim  ;  J.Y. Kim  ;  E.J. Kim  ;  J.G. Lee  ;  D.J. Joo  ;  K.H. Huh  ;  M.S. Kim  ;  Y.S. Kim 
Citation
 Transplantation Proceedings, Vol.48(4) : 1270-1274, 2016 
Journal Title
 Transplantation Proceedings 
ISSN
 0041-1345 
Issue Date
2016
MeSH
4-1BB Ligand/metabolism* ; Animals ; Cell Proliferation/drug effects ; Flow Cytometry ; Glucocorticoid-Induced TNFR-Related Protein/metabolism* ; Immunosuppressive Agents/pharmacology* ; Lymphocyte Activation/drug effects ; Male ; Mice, Inbred C57BL ; Receptors, OX40/metabolism* ; Receptors, Tumor Necrosis Factor/metabolism ; Spleen/immunology ; T-Lymphocyte Subsets/drug effects* ; T-Lymphocyte Subsets/immunology ; T-Lymphocytes, Regulatory/drug effects ; T-Lymphocytes, Regulatory/immunology ; Thalidomide/pharmacology*
Abstract
BACKGROUND: Thalidomide (TM) is known to have anti-cancer and anti-inflammatory properties; however, its mechanism on T cells is still unclear. We previously showed the immune modulatory effect of TM on T cells and its therapeutic effect on lupus nephritis models. Here we examined the changes in the expression of tumor necrosis factor receptor superfamilies (TNFRSFs), including OX40, 4-1BB, and glucocorticoid-induced TNFR-related protein (GITR) in T cell subsets by TM treatments. METHODS: Splenic naïve T cells (Tnaives) from C57BL/6 mice were sort-purified and cultured for CD4(+) T cell proliferation and regulatory T cells (Tregs) conversion with TM treatments. All samples were analyzed by flow cytometry after stained with anti-mouse CD4, Foxp3, OX40, 4-1BB, or GITR antibodies. RESULTS: Expressions of OX40, 4-1BB, and GITR on CD4(+) T cells showed a decreasing tendency by TM treatments. Especially, downregulation of these molecules on CD4(+)CFSE(low) T cells was significant in TM treatment groups. On the condition of Treg conversion, OX40 was downregulated significantly. In contrast, the expression of GITR was increased, and that of 4-1BB had shown no particular change under the condition of Treg. CONCLUSION: Considering these results, TM may have an immune modulatory role through the T cell subset-specific change of OX40, 4-1BB, and GITR expression. Further study is required to elucidate the effect of thalidomide on T cells.
Full Text
http://www.sciencedirect.com/science/article/pii/S0041134516001895
DOI
10.1016/j.transproceed.2015.12.088
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
김명수(Kim, Myoung Soo) ORCID logo https://orcid.org/0000-0002-8975-8381
김범석(Kim, Beom Seok) ORCID logo https://orcid.org/0000-0002-5732-2583
김유선(Kim, Yu Seun) ORCID logo https://orcid.org/0000-0002-5105-1567
이재근(Lee, Jae Geun) ORCID logo https://orcid.org/0000-0002-6722-0257
주동진(Joo, Dong Jin) ORCID logo https://orcid.org/0000-0001-8405-1531
허규하(Huh, Kyu Ha) ORCID logo https://orcid.org/0000-0003-1364-6989
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146979
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