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Distinctive expression patterns of hypoxia-inducible factor-1α and endothelial nitric oxide synthase following hypergravity exposure.

Authors
 Gun Yoon  ;  Choong Sik Oh  ;  Hyun-Soo Kim 
Citation
 ONCOTARGET , Vol.7(23) : 33675-33688, 2016 
Journal Title
ONCOTARGET
Issue Date
2016
MeSH
Animals ; Hypergravity/adverse effects* ; Hypoxia/etiology ; Hypoxia/metabolism* ; Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis* ; Liver/metabolism ; Mice ; Mice, Inbred ICR ; Myocardium/metabolism ; Nitric Oxide Synthase Type III/biosynthesis*
Keywords
Pathology Section ; endothelial nitric oxide synthase ; heart ; hypergravity ; hypoxia-inducible factor-1α ; liver
Abstract
This study was designed to examine the expression of hypoxia-inducible factor-1α (HIF-1α) and the level and activity of endothelial nitric oxide synthase (eNOS) in the hearts and livers of mice exposed to hypergravity. Hypergravity-induced hypoxia and the subsequent post-exposure reoxygenation significantly increased cardiac HIF-1α levels. Furthermore, the levels and activity of cardiac eNOS also showed significant increase immediately following hypergravity exposure and during the reoxygenation period. In contrast, the expression of phosphorylated Akt (p-Akt) and phosphorylated extracellular signal-regulated kinase (p-ERK) showed significant elevation only during the reoxygenation period. These data raise the possibility that the increase in cardiac HIF-1α expression induced by reoxygenation involves a cascade of signaling events, including activation of the Akt and ERK pathways. In the liver, HIF-1α expression was significantly increased immediately after hypergravity exposure, indicating that hypergravity exposure to causes hepatocellular hypoxia. The hypergravity-exposed livers showed significantly higher eNOS immunoreactivity than did those of control mice. Consistent with these results, significant increases in eNOS activity and nitrate/nitrite levels were also observed. These findings suggest that hypergravity-induced hypoxia plays a significant role in the upregulation of hepatic eNOS.
Files in This Item:
T201601874.pdf Download
DOI
10.18632/oncotarget.9372
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun-Soo(김현수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146969
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