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Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds

Authors
 Min Jeong Kim  ;  Yong Cheol Shin  ;  Jong Ho Lee  ;  Seung Won Jun  ;  Chang-Seok Kim  ;  Yunki Lee  ;  Jong-Chul Park  ;  Soo-Hong Lee  ;  Ki Dong Park  ;  Dong-Wook Han 
Citation
 Biomaterials Research, Vol.20 : 2, 2016 
Journal Title
 Biomaterials Research 
ISSN
 2055-7124 
Issue Date
2016
Keywords
3D scaffolds ; C2C12 myoblast ; Hydrogel ; Multiphoton microscopy ; Myogenic differentiation
Abstract
BACKGROUND: Hydrogels can serve as three-dimensional (3D) scaffolds for cell culture and be readily injected into the body. Recent advances in the image technology for 3D scaffolds like hydrogels have attracted considerable attention to overcome the drawbacks of ordinary imaging technologies such as optical and fluorescence microscopy. Multiphoton microscopy (MPM) is an effective method based on the excitation of two-photons. In the present study, C2C12 myoblasts differentiated in 3D gelatin hydroxyphenylpropionic acid (GHPA) hydrogels were imaged by using a custom-built multiphoton excitation fluorescence microscopy to compare the difference in the imaging capacity between conventional microscopy and MPM. RESULTS: The physicochemical properties of GHPA hydrogels were characterized by using scanning electron microscopy and Fourier-transform infrared spectroscopy. In addition, the cell viability and proliferation of C2C12 myoblasts cultured in the GHPA hydrogels were analyzed by using Live/Dead Cell and CCK-8 assays, respectively. It was found that C2C12 cells were well grown and normally proliferated in the hydrogels. Furthermore, the hydrogels were shown to be suitable to facilitate the myogenic differentiation of C2C12 cells incubated in differentiation media, which had been corroborated by MPM. It was very hard to get clear images from a fluorescence microscope. CONCLUSIONS: Our findings suggest that the gelatin-based hydrogels can be beneficially utilized as 3D scaffolds for skeletal muscle engineering and that MPM can be effectively applied to imaging technology for tissue regeneration.
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DOI
10.1186/s40824-016-0050-x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146758
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