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IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation

Authors
 Ji Won Um  ;  Gayoung Choii  ;  Dongseok Park  ;  Dongwook Kim  ;  Sangmin Jeo  ;  Hyeyeon Kang  ;  Takuma Mori  ;  Theofilos Papadopoulos  ;  Taesun Yoo  ;  Yeunkum Lee  ;  Eunjoon Kim  ;  Katsuhiko Tabuchi  ;  X Jaewon Ko 
Citation
 JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.291(19) : 10119-10130, 2016 
Journal Title
 JOURNAL OF BIOLOGICAL CHEMISTRY 
ISSN
 0021-9258 
Issue Date
2016
MeSH
Animals ; Carrier Proteins*/genetics ; Carrier Proteins*/metabolism ; Guanine Nucleotide Exchange Factors*/genetics ; Guanine Nucleotide Exchange Factors*/metabolism ; Humans ; Membrane Proteins*/genetics ; Membrane Proteins*/metabolism ; Rats ; Synapses*/genetics ; Synapses*/metabolism
Keywords
BRAG3 ; IQSEC3 ; SynARFGEF ; gephyrin ; inhibition mechanism ; inhibitory synapse ; protein-protein interaction ; scaffold protein ; synapse ; γ-aminobutyric acid (GABA)
Abstract
Gephyrin is a central scaffold protein that mediates development, function, and plasticity of mammalian inhibitory synapses by interacting with various inhibitory synaptic proteins. Here, we show that IQSEC3, a guanine nucleotide exchange factor for ARF6, directly interacts with gephyrin, an interaction that is critical for the inhibitory synapse localization of IQSEC3. Overexpression of IQSEC3 increases inhibitory, but not excitatory, synapse density in a guanine nucleotide exchange factor activity-dependent manner. Conversely, knockdown of IQSEC3 decreases size of gephyrin cluster without altering gephyrin puncta density. Collectively, these data reveal that IQSEC3 acts together with gephyrin to regulate inhibitory synapse development.
Full Text
http://www.jbc.org/content/291/19/10119
DOI
10.1074/jbc.M115.712893
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Um, Ji Won(엄지원)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146746
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