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PKCδ activated by c-MET enhances infiltration of human glioblastoma cells through NOTCH2 signaling

 Eunji Hwang  ;  Ki-Chun Yoo  ;  Seok-Gu Kang  ;  Rae-Kwon Kim  ;  Yan-Hong Cui  ;  Hae-June Lee  ;  Min-Jung Kim  ;  Jae-Seong Lee  ;  In-Gyu Kim  ;  Yongjoon Suh  ;  Su-Jae Lee 
 ONCOTARGET , Vol.7(4) : 4890-4902, 2016 
Journal Title
Issue Date
Aged ; Animals ; Apoptosis ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism* ; Blotting, Western ; Cell Movement ; Cell Proliferation ; Female ; Glioblastoma/genetics ; Glioblastoma/metabolism ; Glioblastoma/pathology* ; Humans ; Immunoenzyme Techniques ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Protein Kinase C-delta/genetics ; Protein Kinase C-delta/metabolism* ; Proto-Oncogene Proteins c-met/genetics ; Proto-Oncogene Proteins c-met/metabolism* ; RNA, Messenger/genetics ; Real-Time Polymerase Chain Reaction ; Receptor, Notch2/genetics ; Receptor, Notch2/metabolism* ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays
NOTCH2 ; PKCδ ; c-MET ; glioblastoma ; infiltration
Poor prognosis of glioblastoma (GBM) is attributable to the propensity of tumor cells to infiltrate into the brain parenchyma. Protein kinase C (PKC) isozymes are highly expressed or aberrantly activated in GBM. However, how this signaling node translates to GBM cell invasiveness remains unknown. Here, we report that among PKC isoforms, PKCδ is strongly associated with infiltration of GBM cells. Notably, PKCδ enhanced Tyr418 phosphorylation of the non-receptor tyrosine kinase SRC, which in turn activated STAT3 and subsequent NOTCH2 signaling, ultimately leading to GBM cell invasiveness. Furthermore, we showed that PKCδ was aberrantly activated in GBM cells by c-MET, a receptor tyrosine kinase hyperactivated in GBM. In agreement, inhibition either component in the c-MET/PKCδ/SRC/STAT3 signaling axis effectively blocked the NOTCH2 signaling and invasiveness of GBM cells. Taken together, our findings shed a light on the signaling mechanisms behind the constitutive activation of PKCδ signaling in GBM.
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1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
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