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Cholera Toxin Production Induced upon Anaerobic Respiration is Suppressed by Glucose Fermentation in Vibrio cholerae

Authors
 Young Taek Oh  ;  Kang-Mu Lee  ;  Wasimul Bari  ;  Hwa Young Kim  ;  Hye Jin Kim  ;  Sang Sun Yoon 
Citation
 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.26(3) : 627-636, 2016 
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN
 1017-7825 
Issue Date
2016
MeSH
Anaerobiosis ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cholera/microbiology* ; Cholera Toxin/metabolism* ; Fermentation ; Gene Expression Regulation, Bacterial ; Glucose/metabolism* ; Humans ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Vibrio cholerae/genetics ; Vibrio cholerae/growth & development ; Vibrio cholerae/metabolism*
Keywords
Anaerobic respiration ; Cholera toxin ; Glucose metabolism ; Vibrio cholerae
Abstract
The causative agent of pandemic cholera, Vibrio cholerae, infects the anaerobic environment of the human intestine. Production of cholera toxin (CT), a major virulence factor of V. cholerae, is highly induced during anaerobic respiration with trimethylamine N-oxide (TMAO) as an alternative electron acceptor. However, the molecular mechanism of TMAO-stimulated CT production is not fully understood. Herein, we reveal that CT production during anaerobic TMAO respiration is affected by glucose fermentation. When the seventh pandemic V. cholerae O1 strain N16961 was grown with TMAO and additional glucose, CT production was markedly reduced. Furthermore, an N16961 Δcrp mutant, devoid of cyclic AMP receptor protein (CRP), was defective in CT production during growth by anaerobic TMAO respiration, further suggesting a role of glucose metabolism in regulating TMAO-mediated CT production. TMAO reductase activity was noticeably decreased when grown together with glucose or by mutation of the crp gene. A CRP binding region was identified in the promoter region of the torD gene, which encodes a structural subunit of the TMAO reductase. Gel shift assays further confirmed the binding of purified CRP to the torD promoter sequence. Together, our results suggest that the bacterial ability to respire using TMAO is controlled by CRP, whose activity is dependent on glucose availability. Our results reveal a novel mechanism for the regulation of major virulence factor production by V. cholerae under anaerobic growth conditions.
Files in This Item:
T201601012.pdf Download
DOI
10.4014/jmb.1512.12039
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146643
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