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Vasculoprotective Effects of 3-Hydroxybenzaldehyde against VSMCs Proliferation and ECs Inflammation

Authors
 Byung Soo Kong  ;  Soo Jung Im  ;  Yang Jong Lee  ;  Yoon Hee Cho  ;  Yu Ri Do  ;  Jung Woo Byun  ;  Cheol Ryong Ku  ;  Eun Jig Lee 
Citation
 PLOS ONE, Vol.11(3) : e0149394, 2016 
Journal Title
 PLOS ONE 
Issue Date
2016
MeSH
Animals ; Benzaldehydes/pharmacology* ; Carotid Artery Injuries/metabolism ; Catechols/pharmacology ; Cell Movement/drug effects ; Cell Proliferation/drug effects* ; Cells, Cultured ; G1 Phase/drug effects ; Human Umbilical Vein Endothelial Cells/drug effects* ; Human Umbilical Vein Endothelial Cells/metabolism ; Humans ; Inflammation/drug therapy* ; Inflammation/metabolism ; Intercellular Adhesion Molecule-1/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/metabolism ; Myocytes, Smooth Muscle/drug effects ; Myocytes, Smooth Muscle/metabolism ; NF-kappa B/metabolism ; Neointima/drug therapy ; Neointima/metabolism ; Phosphorylation/drug effects ; Proto-Oncogene Proteins c-akt/metabolism ; Rats ; Rats, Sprague-Dawley ; Resting Phase, Cell Cycle/drug effects ; Vascular Cell Adhesion Molecule-1/metabolism
Abstract
3-hydroxybenzaldehyde (3-HBA) is a precursor compound for phenolic compounds like Protocatechuic aldehyde (PCA). From recent reports, PCA has shown vasculoprotective potency, but the effects of 3-HBA remain unclear. The aim of this study is to investigate the vasculoprotective effects of 3-HBA in endothelial cells, vascular smooth muscle cells and various animal models. We tested effects of 3-HBA in both vitro and vivo. 3-HBA showed that it prevents PDGF-induced vascular smooth muscle cells (VSMCs) migration and proliferation from MTS, BrdU assays and inhibition of AKT phosphorylation. It arrested S and G0/G1 phase of VSMC cell cycle in PI staining and it also showed inhibited expression levels of Rb1 and CD1. In human umbilical vein endothelial cells (HUVECs), 3-HBA inhibited inflammatory markers and signaling molecules (VCAM-1, ICAM-1, p-NF-κB and p-p38). For ex vivo, 3-HBA has shown dramatic effects in suppressing the sprouting from aortic ring of Spargue Dawley (SD) rats. In vivo data supported the vasculoprotective effects of 3-HBA as it inhibited angiogenesis from Matrigel Plug assay in C57BL6 mouse, prevented ADP-induced thrombus generation, increased blood circulation after formation of thrombus, and attenuated neointima formation induced by common carotid artery balloon injury of SD rats. 3-HBA, a novel therapeutic agent, has shown vasculoprotective potency in both in vitro and in vivo.
Files in This Item:
T201600904.pdf Download
DOI
10.1371/journal.pone.0149394
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Endocrinology (내분비연구소) > 1. Journal Papers
Yonsei Authors
Ku, Cheol Ryong(구철룡) ORCID logo https://orcid.org/0000-0001-8693-9630
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
Cho, Yoon Hee(조윤희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146581
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