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TSLP Is a Potential Initiator of Collagen Synthesis and an Activator of CXCR4/SDF-1 Axis in Keloid Pathogenesis

 Jung U Shin  ;  Seo Hyeong Kim  ;  Hyeran Kim  ;  Ji Yeon Noh  ;  Shan Jin  ;  Chang Ook Park  ;  Won Jai Lee  ;  Dong Won Lee  ;  Ju Hee Lee  ;  Kwang Hoon Lee 
 Journal of Investigative Dermatology, Vol.136(2) : 507-515, 2016 
Journal Title
 Journal of Investigative Dermatology 
Issue Date
Animals ; Blotting, Western ; Cells, Cultured ; Chemokine CXCL12/metabolism ; Collagen/biosynthesis* ; Cytokines/metabolism* ; Cytokines/pharmacology ; Disease Models, Animal ; Female ; Fibroblasts/metabolism* ; Fluorescent Antibody Technique ; Humans ; Immunohistochemistry ; Keloid/pathology* ; Keloid/physiopathology ; Keloid/therapy ; Mice ; Mice, Inbred BALB C ; Real-Time Polymerase Chain Reaction/methods ; Receptors, CXCR4/metabolism* ; Sampling Studies
Recently, thymic stromal lymphopoietin (TSLP), which is well studied in allergic diseases, has been reported in fibrotic diseases, including idiopathic pulmonary fibrosis and atopic dermatitis fibrosis. However, the role of TSLP in keloid is obscure. In this study, we assessed the expression of TSLP in keloid tissue and investigated the possible role of TSLP in keloid pathogenesis. We observed that TSLP expression was increased in keloid tissue compared to normal tissue. Furthermore, TSLP treatment induced increased collagen I and collagen III expression in fibroblasts via transforming growth factor-?; however, there was higher expression in keloid fibroblasts compared to normal fibroblasts. Stromal cell-derived factor-1?, which was recently reported to enhance wound healing through recruiting bone marrow-derived mesenchymal stem cells to the wound area, increased after TSLP treatment in fibroblasts and was primarily expressed in ?-smooth muscle action-positive myofibroblasts in keloid tissue. Furthermore, fibrocytes expressing CXCR4, a stromal cell-derived factor-1? receptor, were significantly increased in keloid tissue compared to normal tissue. Finally, intradermal TSLP injection on BALB/c mice increased stromal cell-derived factor-1? expression and CXCR4(+) fibrocytes infiltration. Our data suggest that TSLP is a potent inducer of collagen and transforming growth factor-? production in keloid fibroblasts. In addition, it might activate the CXCR4/stromal cell-derived factor-1 axis to increase fibrocyte infiltration into the keloid tissue.
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Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Noh, Ji Yeon(노지연)
Park, Chang Ook(박창욱) ORCID logo https://orcid.org/0000-0003-3856-1201
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Lee, Kwang Hoon(이광훈)
Lee, Dong Won(이동원) ORCID logo https://orcid.org/0000-0003-0046-3139
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
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