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Effect of Pneumoperitoneum on Oxidative Stress and Inflammation via the Arginase Pathway in Rats

Authors
 Seokyung Shin  ;  Sungwon Na  ;  Ok Soo Kim  ;  Yong Seon Choi  ;  Shin Hyung Kim  ;  Young Jun Oh 
Citation
 Yonsei Medical Journal, Vol.57(1) : 238-246, 2016 
Journal Title
 Yonsei Medical Journal 
ISSN
 0513-5796 
Issue Date
2016
MeSH
Animals ; Aorta/physiology* ; Arginase/antagonists & inhibitors* ; Enzyme Inhibitors/administration & dosage ; Enzyme Inhibitors/pharmacology ; Inflammation/etiology ; Inflammation/prevention & control* ; Injections, Subcutaneous ; Lung Injury/etiology ; Lung Injury/prevention & control ; Male ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/metabolism* ; Nitric Oxide Synthase Type III/metabolism* ; Oxidative Stress/drug effects* ; Pneumoperitoneum/complications* ; Pneumoperitoneum/drug therapy ; Rats ; Rats, Sprague-Dawley
Keywords
Arginase ; nitric oxide synthase ; oxidative stress ; pneumoperitoneum
Abstract
PURPOSE: Oxidative stress during CO₂ pneumoperitoneum is reported to be associated with decreased bioactivity of nitric oxide (NO). However, the changes in endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), and arginase during CO₂ pneumoperitoneum have not been elucidated. MATERIALS AND METHODS: Thirty male Sprague-Dawley rats were randomized into three groups. After anesthesia induction, the abdominal cavities of the rats of groups intra-abdominal pressure (IAP)-10 and IAP-20 were insufflated with CO₂ at pressures of 10 mm Hg and 20 mm Hg, respectively, for 2 hours. The rats of group IAP-0 were not insufflated. After deflation, plasma NO was measured, while protein expression levels and activity of eNOS, iNOS, arginase (Arg) I, and Arg II were analyzed with aorta and lung tissue samples. RESULTS: Plasma nitrite concentration and eNOS expression were significantly suppressed in groups IAP-10 and IAP-20 compared to IAP-0. While expression of iNOS and Arg I were comparable between the three groups, Arg II expression was significantly greater in group IAP-20 than in group IAP-0. Activity of eNOS was significantly lower in groups IAP-10 and IAP-20 than in group IAP-0, while iNOS activity was significantly greater in group IAP-20 than in groups IAP-0 and IAP-10. Arginase activity was significantly greater in group IAP-20 than in groups IAP-0 and IAP-10. CONCLUSION: The activity of eNOS decreases during CO₂ pneumoperitoneum, while iNOS activity is significantly increased, a change that contributes to increased oxidative stress and inflammation. Moreover, arginase expression and activity is increased during CO₂ pneumoperitoneum, which seems to act inversely to the NO system.
Files in This Item:
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DOI
10.3349/ymj.2016.57.1.238
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
김신형(Kim, Shin Hyung) ORCID logo https://orcid.org/0000-0003-4058-7697
나성원(Na, Sungwon) ORCID logo https://orcid.org/0000-0002-1170-8042
신서경(Shin, Seokyung) ORCID logo https://orcid.org/0000-0002-2641-0070
오영준(Oh, Young Jun) ORCID logo https://orcid.org/0000-0002-6258-5695
최용선(Choi, Yong Seon) ORCID logo https://orcid.org/0000-0002-5348-864X
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146315
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