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TGF-β induced apoptosis and reduction of Bcl-2 in human lens epithelial cells in vitro

Authors
 Joon H. Lee  ;  Xiu Hua Wan  ;  Jeongmin Song  ;  Jimmy Jaeyoung Kang  ;  Woo Sook Chung  ;  Eunjoo H. Lee  ;  Eung Kweon Kim 
Citation
 CURRENT EYE RESEARCH, Vol.25(3) : 147-153, 2002 
Journal Title
 CURRENT EYE RESEARCH 
ISSN
 0271-3683 
Issue Date
2002
Keywords
apoptosis ; Bcl-2 ; lens epithelium ; TGF-b
Abstract
Purpose. TGF-ß has been shown to induce lens epithelial cells to undergo an aberrant growth and transdifferentiation that mimic some characteristics of anterior subcapsular cataract, and posterior capsular opacification. Here we sought to examine whether TGF-ß induces apoptotic cell death in human lens epithelial cells in vitro. Methods. Cell death of human lens epithelial cells HLE-B3 cell line was measured by TUNEL assay, FACS analysis, and DNA fragmentation assay. The expression of Bcl-2 and Bax was examined using reverse transcription-polymerase chain reaction and Western blot analysis. The expression of poly (ADP-ribose) polymerase (PARP) and caspase-3 was examined using Western blot analysis. Results. TGF-ß-induced cell death was detected in human lens epithelial cells by TUNEL assay. DNA fragmentation assay showed the characteristic laddering pattern from the genomic DNA from human lens epithelial cells treated with TGF-ß. The expression of Bcl-2 mRNA and its protein was markedly decreased in human lens epithelial cells treated with TGF-ß. Caspase-3 and PARP were not activated in this process. Conclusions. This study suggests that TGF-ß induces apoptotic cell death in human lens epithelial cells and that decreased expression of Bcl-2 might play a role in this process.
Full Text
http://www.tandfonline.com/doi/abs/10.1076/ceyr.25.3.147.13475#.VektZT8w_cs
DOI
10.1076/ceyr.25.3.147.13475
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144357
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