0 529

Cited 2 times in

Isolation of genes induced in Naegleria fowleriduring mouse brain passage

Authors
 Ho-Cheol Yun  ;  Soon-Jung Park  ;  Hyun-Hee Kong  ;  Dong-Il Chung 
Citation
 EUROPEAN JOURNAL OF PROTISTOLOGY, Vol.38(2) : 105-111, 2002 
Journal Title
EUROPEAN JOURNAL OF PROTISTOLOGY
ISSN
 0932-4739 
Issue Date
2002
Keywords
Naegleria fowleri ; differential gene expression ; mouse brain passage
Abstract
We have isolated genes that are differentially expressed between Naegleria fowleri and N. gruberi by reverse dot blot analysis. Northern blot hybridization was applied to compare the expression patterns of the isolated genes before and after restoration of virulence by mouse brain passage of N. fowleri. Four hundred and eighty randomly selected cDNA clones of N. fowleri were hybridized with single-stranded cDNA probes of N. fowleri or N. gruberi. Among them, thirty-two clones showed a different signal between the two species. We determined the nucleotide sequences of twenty-two clones that displayed stronger hybridization with cDNA probes of N. fowleri than of N. gruberi. Only six clones provided useful information on their putative functions by sequence homology search through public databases. Four of them, showing a constant level of expression during mouse brain passage, encoded ribosomal protein, fumarase, malate dehydrogenase, or ubiquitin. The remaining two clones were homologous to genes for the high mobility group (HMG) protein and the 26S proteasome subunit. Interestingly, the expressions of these two genes were increased by successive mouse brain passages of N. fowleri. These results suggested that the HMG protein and 26S proteasome of N. fowleri may be involved in some biological processes to adapt the amoeba as a pathogen to the host.
Full Text
http://www.sciencedirect.com/science/article/pii/S093247390470053X
DOI
10.1078/0932-4739-00859
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144202
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links