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Identification of MARCKS, FLJ11383 and TAF1B as putative novel target genes in colorectal carcinomas with mocrosatellite instability

 Nam Gyun Kim  ;  Hwanseok Rhee  ;  Long Shan Li  ;  Hyunki Kim  ;  Jin Sung Lee  ;  Joo Hang Kim  ;  Nam Kyu Kim  ;  Hoguen Kim 
 ONCOGENE, Vol.21(33) : 5081-5087, 2002 
Journal Title
Issue Date
Base Sequence ; Colorectal Neoplasms/genetics* ; DNA Mutational Analysis ; DNA, Neoplasm/genetics ; DNA-Binding Proteins/genetics* ; Databases, Nucleic Acid ; Frameshift Mutation ; Glucosidases ; Humans ; Intracellular Signaling Peptides and Proteins* ; Membrane Proteins* ; Microsatellite Repeats/genetics* ; Molecular Sequence Data ; Mutagenesis/genetics* ; Myristoylated Alanine-Rich C Kinase Substrate ; Neoplasm Proteins/genetics* ; Phosphoproteins/genetics* ; Polymerase Chain Reaction ; Terminal Repeat Sequences ; Tumor Cells, Cultured
Somatic frameshift mutations in some genes containing coding mononucleotide repeats (cMNRs) are well known characteristics of tumors with high microsatellite instability (MSI-H). We identified 22 novel and 11 known target genes containing cMNRs with a length of 10 or more nucleotides by using a systematic database search. Frameshift mutation analysis was performed with these 33 genes in 39 MSI-H and 24 microsatellite stable (MSS) colorectal carcinomas by assessing the mobility shifts of PCR products in gel electrophoresis and by sequencing. All the 39 MSI-H colorectal carcinomas, except one, showed mutations in more than one gene, while no mutations were found in 24 MSS colorectal carcinomas. Of these MSI-H tumors, 11 genes were mutated in more than 40%. The most frequently mutated novel genes were MARCKS (72%), FLJ11383 (74%) and TAF1B (82%). Biallelic inactivation in MARCKS and FLJ11383 was also frequent in MSI-H tumors. The observed mutation frequency of the 11 known target genes was compatible with that found by previous studies. The very high frequency of mutations, biallelic mutations and the predicted truncation of protein products suggests that mutations of MARCKS, FLJ11383 and TAF1B are selected, and play a role in the tumorigenesis of MSI-H colorectal carcinomas.
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10.1038/sj.onc. 1205703
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Joo Hang(김주항)
Kim, Ho Keun(김호근)
Lee, Jin Sung(이진성) ORCID logo https://orcid.org/0000-0002-1262-8597
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