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Endotoxin-neutralizing antimicrobial proteins of the human placenta

 Hun Sik Kim  ;  Ju Hyun Cho  ;  Hyoung Woo Park  ;  Ho Yoon  ;  Mi Sun Kim  ;  Sun Chang Kim 
 JOURNAL OF IMMUNOLOGY, Vol.168(5) : 2356-2364, 2002 
Journal Title
Issue Date
Amino Acid Sequence ; Amnion/cytology ; Amnion/metabolism ; Amniotic Fluid/physiology ; Anti-Infective Agents/chemistry ; Anti-Infective Agents/isolation & purification ; Anti-Infective Agents/pharmacology* ; Cell Line ; Epithelial Cells/metabolism ; Histones/chemistry ; Histones/isolation & purification ; Histones/pharmacology* ; Humans ; Immunohistochemistry ; Lipopolysaccharides/antagonists & inhibitors* ; Lipopolysaccharides/metabolism ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Placenta/chemistry* ; Salts/chemistry ; Trophoblasts/metabolism
Microbial colonization and infection of placental tissues often lead to adverse pregnancy outcomes such as preterm birth, a leading cause of neonatal morbidity and mortality. The fetal membranes of the placenta, a physical and active barrier to microbial invasion, encapsulate the fetus and secure its intrauterine environment. To examine the innate defense system of the human placenta, antimicrobial peptides were isolated from the fetal membranes of human placenta and characterized biochemically. Two salt-resistant antimicrobial host proteins were purified to homogeneity using heparin-affinity and reversed-phase HPLC. Characterization of these proteins revealed that they are identical to histones H2A and H2B. Histones H2A and H2B showed dosedependent inhibition of the endotoxin activity of LPS and inhibited this activity by binding to and therefore blocking both the core and lipid A moieties of LPS. Consistent with a role for histones in the establishment of placental innate defense, histones H2A and H2B were highly expressed in the cytoplasm of syncytiotrophoblasts and amnion cells, where the histone proteins were localized mainly to the epithelial surface. Furthermore, culturing of amnion-derived WISH cells led to the constitutive release of histone H2B, and histones H2A and H2B contribute to bactericidal activity of amniotic fluid. Our studies suggest that histones H2A and H2B may endow the epithelium of the placenta with an antimicrobial and endotoxin-neutralizing barrier against microorganisms that invade this immune-privileged site. The Journal of Immunology, 2002, 168: 2356–2364.
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1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Park, Hyoung Woo(박형우) ORCID logo https://orcid.org/0000-0002-5341-9249
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