Animals ; Base Sequence ; China ; DNA Primers ; Feces/parasitology ; Genes, Protozoan* ; Giardia lamblia/classification ; Giardia lamblia/genetics* ; Giardiasis/parasitology ; Humans ; Intestinal Diseases, Parasitic/parasitology ; Korea ; Laos ; Molecular Sequence Data ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length* ; Protozoan Proteins/genetics* ; Sequence Alignment ; Species Specificity
Keywords
Giardia intestinalis ; GLORF-C4 ; PCR-RFLP
Abstract
A cDNA clone encoding GLORF-C4 was isolated from the WB strain, an assemblage A Giardia intestinalis. Interestingly, GLORF-G4 has been previously reported as an assemblage B-specific gene. Using two primers based on GLORF-C4 of the GS strain, a prototype assemblage B, GLORFG4 gene was amplified from all the groups of G. intestinalis, and applied to detect the presence of cysts of G. intestinalis from faecal samples of cyst-passers. RFLP analysis of this PCR product successfully classified G. intestinalis into two distinct groups, assemblages A and B.