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Expression Patterns of Cytokines and Chemokines Genes in Human Hepatoma Cells

 Eui Cheol Shin  ;  Youn Hee Choi  ;  Ji Su Kim  ;  Se Jong Kim  ;  Jeon Han Park 
 YONSEI MEDICAL JOURNAL, Vol.43(5) : 657-664, 2002 
Journal Title
Issue Date
Carcinoma, Hepatocellular/metabolism* ; Chemokines/genetics* ; Cytokines/genetics* ; Gene Expression Profiling ; Humans ; Liver Neoplasms/metabolism* ; RNA, Messenger/analysis ; Tumor Cells, Cultured ; fas Receptor/physiology
Hepatoma ; cytokine ; chemokine ; TGF-β ; interleukin-8 ; MCP-1 ; Fas
Various cytokines and chemokines play a role in carcinogenesis. However, no study has previously been undertaken to investigate comprehensively the expressions of cytokines and chemokines in hepatoma cells. In this study, we determined which cytokines and chemokines are expressed in hepatoma cells. Recently, it was reported that the expressions of several chemokines could be increased by Fas stimulus in many normal and cancer cells. Therefore, we also investigated whether chemokines expression is regulated by Fas ligation. To address this issue, we performed RNase protection assays upon 13 cytokines and 8 chemokines genes in 10 human hepatoma cell lines, comprising 8 hepatitis B virus (HBV)-associated hepatoma cell lines. Transforming growth factor-β2 (TGF-β2) was found to be expressed in 8 HBV-associated hepatoma cell lines, and to be potently expressed in 5 cell lines; however, the mRNA expressions of interleukin-10 (IL-10), IL-12, interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) were not detected in any cell lines examined. Among the chemokines investigated in this study, IL-8 was expressed by 8 HBV- associated hepatoma cell lines, and monocyte chemoattractant protein-1 (MCP-1) by 7 HBV-associated hepatoma cell lines. However, the mRNA expressions of macrophage inflammatory protein-1α (MIP-1α), MIP-β, interferon-inducible protein-10 (IP-10), RANTES, lymphotactin and I-309 were either very weak or undetectable. Fas ligation did not increase chemokines expression in hepatoma cells. Conclusively, TGF-β2, IL-8 and MCP-1 were overexpressed in HBV-associated hepatoma cells, and the expressions of chemokines were not increased by Fas ligation in human hepatoma cells.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
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