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Evaluation of E1b gene-attenuated replicating adenoviruses for cancer gene therapy

 Jaesung Kim  ;  Jae Yong Cho  ;  Joo Hang Kim  ;  Kyeong Cheon Jung  ;  Chae Ok Yun 
 CANCER GENE THERAPY, Vol.9(9) : 725-736, 2002 
Journal Title
Issue Date
Adenoviridae/physiology* ; Adenovirus E1B Proteins/genetics* ; Adenovirus E1B Proteins/metabolism ; Animals ; Blotting, Western ; Cell Division ; Colony-Forming Units Assay ; DNA Primers/chemistry ; DNA, Viral/metabolism ; Female ; Gene Deletion ; Genetic Therapy* ; Genetic Vectors* ; Humans ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Neoplasms, Experimental/pathology ; Neoplasms, Experimental/therapy* ; Paclitaxel/pharmacology ; Polymerase Chain Reaction ; Tumor Cells, Cultured ; Viral Plaque Assay ; Virus Replication*
Gene-attenuated replication-competent adenoviruses are emerging as a promising new modality for the treatment of cancer. For the aim of improving adenoviral vectors for cancer gene therapy, we have constructed genetically attenuated adenoviral vectors with different combinations of E1B genes and investigated the possibility of enhanced oncolytic and replication effects of these engineered replication-competent adenoviruses. We show here that the cytolytic potency of each gene-attenuated replicating adenovirus differed significantly depending on the presence or deletion of E1B 55 kDa and E1B 19 kDa function. More specifically, among the constructed vectors (Ad-ΔE1B19, Ad-ΔE1B55, Ad-ΔE1B19/55, and Ad-wt), E1B 19 kDa–inactivated adenovirus (Ad-ΔE1B19) was the most potent against all tumor cells tested, inducing the largest-sized plaques and marked CPE. Further, cells infected with either Ad-ΔE1B19 or E1B19/55 kDa–deleted adenovirus (Ad-ΔE1B19/55) showed complete cell lysis with disintegrated cellular structure, whereas cells infected with Ad-wt maintained intact cellular and nuclear membrane with properly structured organelles. TUNEL and DNA fragmentation assay also revealed that the Ad-ΔE1B19 or Ad-ΔE1B19/55 adenovirus-infected cells showed more profound induction of apoptosis in comparison to wild-type adenovirus-infected cells. The presence of E1B 55 kDa gene was required for efficient viral replication and deletion of E1B 19 kDa function in replicating adenovirus-induced apoptosis, leading to increased cytopathic effects. Moreover, Ad-ΔE1B19 adenovirus showed a better antitumor effect than other E1B-attenuated adenoviruses. Taken together, the replicating adenoviruses deleted in E1B 19 kDa function may serve as an improved vector for anticancer gene therapy in combination with apoptosis-inducing modalities such as chemotherapeutic agents and radiation therapy.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Joo Hang(김주항)
Yun, Chae Ok(윤채옥)
Cho, Jae Yong(조재용) ORCID logo https://orcid.org/0000-0002-0926-1819
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