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Estrogen regulates a tissue-specific calpain in the anterior pituitary

Authors
 W Rachel Duan  ;  Masafumi Ito  ;  Eun Jig Lee  ;  Pei-Yu Chien  ;  J Larry Jameson 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.295(2) : 261-266, 2002 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2002
MeSH
Animals ; Base Sequence ; Calpain/physiology* ; Cloning, Molecular ; DNA, Complementary ; Estrogens/physiology* ; Female ; Molecular Sequence Data ; Pituitary Gland, Anterior/physiology* ; Promoter Regions, Genetic ; Rats ; Rats, Sprague-Dawley
Keywords
Calpain ; Estrogen ; Pituitary ; Estrogen-response element
Abstract
A PCR-based cDNA subtraction hybridization was performed to identify the genes stimulated by estrogen in the pituitary. A novel tissue-specific calpain (nCL-2′), previously shown to be expressed mainly in the stomach, was markedly induced in the pituitary after estrogen treatment. The 5′-flanking region of the calpain nCL-2′ gene was analyzed to assess the molecular mechanism of estrogen regulation. Sequence analysis of the nCL-2′ promoter (1.9 kb) revealed a perfectly palindromic putative estrogen-response element (ERE), GGTCATGCTGACC. In transient transfection studies, the nCL-2′ promoter was highly responsive to estrogen in the presence of estrogen receptor (ER). Transcriptional activation by estrogen was prevented by an ERE mutation as well as by mutations in the ER DNA-binding domain. An ER antagonist, ICI 182780, blocked estrogen inducibility of the nCL-2′ promoter. We conclude that the nCL-2′ form of calpain is expressed in the pituitary and upregulated by estrogen at the transcription level.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X02006551
DOI
10.1016/S0006-291X(02)00655-1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143759
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