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Estrogen regulates a tissue-specific calpain in the anterior pituitary

DC FieldValueLanguage
dc.contributor.author이은직-
dc.date.accessioned2016-05-16T11:05:17Z-
dc.date.available2016-05-16T11:05:17Z-
dc.date.issued2002-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143759-
dc.description.abstractA PCR-based cDNA subtraction hybridization was performed to identify the genes stimulated by estrogen in the pituitary. A novel tissue-specific calpain (nCL-2′), previously shown to be expressed mainly in the stomach, was markedly induced in the pituitary after estrogen treatment. The 5′-flanking region of the calpain nCL-2′ gene was analyzed to assess the molecular mechanism of estrogen regulation. Sequence analysis of the nCL-2′ promoter (1.9 kb) revealed a perfectly palindromic putative estrogen-response element (ERE), GGTCATGCTGACC. In transient transfection studies, the nCL-2′ promoter was highly responsive to estrogen in the presence of estrogen receptor (ER). Transcriptional activation by estrogen was prevented by an ERE mutation as well as by mutations in the ER DNA-binding domain. An ER antagonist, ICI 182780, blocked estrogen inducibility of the nCL-2′ promoter. We conclude that the nCL-2′ form of calpain is expressed in the pituitary and upregulated by estrogen at the transcription level.-
dc.description.statementOfResponsibilityopen-
dc.format.extent261~266-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHCalpain/physiology*-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHDNA, Complementary-
dc.subject.MESHEstrogens/physiology*-
dc.subject.MESHFemale-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPituitary Gland, Anterior/physiology*-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.titleEstrogen regulates a tissue-specific calpain in the anterior pituitary-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorW Rachel Duan-
dc.contributor.googleauthorMasafumi Ito-
dc.contributor.googleauthorEun Jig Lee-
dc.contributor.googleauthorPei-Yu Chien-
dc.contributor.googleauthorJ Larry Jameson-
dc.identifier.doi10.1016/S0006-291X(02)00655-1-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03050-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid12150941-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X02006551-
dc.subject.keywordCalpain-
dc.subject.keywordEstrogen-
dc.subject.keywordPituitary-
dc.subject.keywordEstrogen-response element-
dc.contributor.alternativeNameLee, Eun Jig-
dc.contributor.affiliatedAuthorLee, Eun Jig-
dc.rights.accessRightsnot free-
dc.citation.volume295-
dc.citation.number2-
dc.citation.startPage261-
dc.citation.endPage266-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.295(2) : 261-266, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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