Sol-Gel derived phosphate water-soluble glasses containing Cu were prepared. Powder-shape of glasses were added in D.I.water used polyethylene bottle. After solution contained glass powder were submerged in water bath on 25℃, their dissolution behavior/characteristics, bactericidal effect and cytotoxicity test were evaluated. The maximum amount of Cu (35 mol%) via sol-gel method was more 5 mol% increased than that with melting process. The stage of total dissolution was more dominant than that of selective leaching during dissolution due to dissolved amount of glasses increased linearly with time. The ratio of Cu+ to Cu2+ was 3 : 7 so that the structure of glasses is more predominant 2-dimension chain structure than 3-dimension polymeric structure. The stage of total dissolution was more dominant than that of selective leaching during dissolution. Bactericidal effect against all bacteria showed that solutions which contained 40 ppm and 100 ppm of Cu killed 80 percentages of bacteria within 2 hours and 100 percentages of those within 12 hours. The result of cytotoxicity test for L929 cells showed no cytotoxicity were observed within 96 hours for dissolved solution that contains 40 ppm and 100 ppm of Cu.