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The Basis for IL-2-Induced IL-2 Receptor α Chain Gene Regulation: Importance of Two Widely Separated IL-2 Response Elements

Authors
 Hyoung-Pyo Kim  ;  John Kelly  ;  Warren J. Leonard 
Citation
 IMMUNITY, Vol.15(1) : 159-172, 2001 
Journal Title
IMMUNITY
ISSN
 1074-7613 
Issue Date
2001
MeSH
Amino Acid Motifs ; Animals ; Base Sequence ; DNA-Binding Proteins/metabolism ; Deoxyribonuclease I/pharmacology ; Gene Expression Regulation/drug effects* ; HMGA1a Protein ; High Mobility Group Proteins/metabolism ; Humans ; Interleukin-2/pharmacology* ; Mice ; Mice, Knockout ; Milk Proteins* ; Molecular Sequence Data ; Promoter Regions, Genetic ; Receptors, Interleukin-2/genetics* ; Response Elements* ; STAT5 Transcription Factor ; Trans-Activators/metabolism ; Transcription Factors/metabolism ; Tumor Suppressor Proteins
Abstract
The interleukin-2 receptor α (IL-2Rα) chain is an essential component of high-affinity IL-2 receptors. Accordingly, IL-2Rα expression helps to regulate T cell growth and other lymphoid functions. Lineage-restricted and activation-dependent IL-2Rα transcription is controlled by three upstream positive regulatory regions (PRRs). We now describe an additional IL-2 response element, PRRIV, within intron 1, in humans and mice. PRRIV activity requires GAS motifs that bind Stat5 proteins and additional upstream HMG-I(Y) binding sites. Moreover, IL-2 induces the binding of HMG-I(Y), Stat5a, and Stat5b in vivo to PRRIV and PRRIII, which also functions as an IL-2 response element. Thus, the IL-2 inducibility of the IL-2Rα gene is unexpectedly mediated by two widely separated regulatory Stat5-dependent elements, located both upstream and downstream of the transcription initiation sites.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyoung Pyo(김형표) ORCID logo https://orcid.org/0000-0003-1441-8822
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143204
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