Purpose : We reported a case of squamous cell carcinoma in a patient with chronic herpetic keratitis treated with Mitomycin C. Methods : In a patient with 13-year recurrent chronic herpetic keratitis, we diagnosed invasive squamous cell carcinoma in papillary mass with no response of previous treatment by conjunctival biopsy. Results : After surgical removal and chemotherapy of 0.04% topical Mitomycin C, the eye showed histopathological resolution of squamous cell carcinoma.