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Improved calcification resistance and biocompatibility of tissue patch grafted with sulfonated PEO or heparin after glutaraldehyde fixation

 Won Kyu Lee  ;  Ki Dong Park  ;  Young Ha Kim  ;  Hwal Suh  ;  Jong Chul Park  ;  Jong Eun Lee  ;  Kyung Sun  ;  Man Jong Baek  ;  Hyung-Mook Kim  ;  Soo Hyun Kim 
 Journal of Biomedical Materials Research, Vol.58(1) : 27-35, 2001 
Journal Title
 Journal of Biomedical Materials Research 
Issue Date
Animals ; Aorta, Thoracic/pathology ; Aorta, Thoracic/surgery ; Biocompatible Materials/chemistry ; Biocompatible Materials/toxicity ; Bioprosthesis* ; Borohydrides/pharmacology ; Calcinosis/prevention & control* ; Cattle ; Collagenases/pharmacology ; Cross-Linking Reagents/chemistry ; Cross-Linking Reagents/pharmacology* ; Cross-Linking Reagents/toxicity ; Dermatologic Surgical Procedures ; Dogs ; Fixatives/chemistry ; Fixatives/pharmacology* ; Fixatives/toxicity ; Glutaral/chemistry ; Glutaral/pharmacology* ; Glutaral/toxicity ; Heparin/chemistry ; Heparin/pharmacology* ; Heparin/toxicity ; L Cells (Cell Line)/drug effects ; Male ; Materials Testing ; Mice ; Oxidation-Reduction ; Pericardium/chemistry ; Pericardium/drug effects* ; Polyethylene Glycols/chemistry ; Polyethylene Glycols/pharmacology* ; Pulmonary Artery/pathology ; Pulmonary Artery/surgery ; Rats ; Rats, Sprague-Dawley ; Skin/pathology ; Specimen Handling ; Sulfonic Acids/chemistry ; Sulfonic Acids/pharmacology* ; Temperature ; Tensile Strength
bovine pericardium tissue patch ; sulfonated PEO or heparin grafting ; stability ; cytotoxicity ; calcification
A novel chemical modification of biological tissues was developed aimed at improving biocompatibility and calcification resistance. This method involved the additional grafting of sulfonated PEO (PEO-SO3) or heparin after conventional glutaraldehyde (GA) fixation of bovine pericardium (BP). The amino groups of PEO-SO3 or heparin were utilized to react to the GA residues to block them. The PEO-SO3 or heparin grafted tissues demonstrated a slightly higher shrinkage temperature and tensile strength, but greater resistance to collagenase digestion, than GA treated ones. These results suggest that modified tissues have improved durability due to the grafting and filling effect of PEO-SO3 or heparin in addition to the GA cross-linking. At the direct contact cytotoxicity test in vitro, PEO-SO3 or heparin grafted tissue was shown to be nontoxic, while relatively significant cytotoxicity was observed for the GA treated tissues, possibly due to the release of GA. From the in vivo calcification study, calcium contents deposited on the modified tissues were much less than those on GA treated tissues. Such a decreased calcification might be explained by the decrease of residual GA groups during the additional treatment, and the space-filling effect and the nonadhesive property and/or the blood compatiblility of PEO-SO3 or heparin grafted covalently. The newly modified tissue patch was observed to show improved pathological assessibility including less inflammation and tissue reactions. This simple modification method may be useful for calcification-resistant and blood-compatible tissue patches for cardiovascular implants.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
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