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Effect of Centrifugal Force on Cellular Activity of Osteoblastic MC3T3-E1 Cells in vitro

Authors
 Dong Hee Lee  ;  Jong Chul Park  ;  Hwal Suh 
Citation
 YONSEI MEDICAL JOURNAL, Vol.42(4) : 405-410, 2001 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
2001
MeSH
Alkaline Phosphatase/metabolism ; Animals ; Cell Division ; Cells, Cultured ; Centrifugation ; DNA/biosynthesis ; Mice ; Osteoblasts/physiology* ; Stress, Mechanical
Keywords
Centrifugation ; osteoblast ; MC3T3-E1 ; alkaline phosphatase ; DNA synthesis ; fetal bovin serum
Abstract
The effects of centrifugal force on growth and differentiation of osteoblastic cells cultured in α-MEM containing 1% Fetal bovine serum were investigated by assays of DNA synthesis, alkaline phosphatase activity and osteocalcin-production in osteoblastic MC3T3-E1 cells. Centrifugation of the cells in low concentrations (1%) of fetal bovine serum caused a 1.9-fold increase of [3H] thymidine incorporation on day 3 from the start of centrifugation, and gradually decreased with culture up to day 9. Alkaline phosphatase activity was not affected by centrifugal force until day 5, and increased rapidly after day 7. Stimulation of DNA synthesis by centrifugation was abolished in the presence of H-7, an inhibitor of protein kinase C. These results suggest that centrifugal force stimulates the proliferation of osteoblastic cells through an autocrine secretion of some diffusable growth-promoting activity. Additional centrifugation of the cells also slightly stimulated alkaline phosphatase activity, although this did not directly influence the cell's osteocalcin-production activity.
Files in This Item:
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DOI
10.3349/ymj.2001.42.4.405
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143163
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