Cited 13 times in
Effect of Centrifugal Force on Cellular Activity of Osteoblastic MC3T3-E1 Cells in vitro
DC Field | Value | Language |
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dc.contributor.author | 박종철 | - |
dc.contributor.author | 서활 | - |
dc.date.accessioned | 2016-02-19T11:26:51Z | - |
dc.date.available | 2016-02-19T11:26:51Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143163 | - |
dc.description.abstract | The effects of centrifugal force on growth and differentiation of osteoblastic cells cultured in α-MEM containing 1% Fetal bovine serum were investigated by assays of DNA synthesis, alkaline phosphatase activity and osteocalcin-production in osteoblastic MC3T3-E1 cells. Centrifugation of the cells in low concentrations (1%) of fetal bovine serum caused a 1.9-fold increase of [3H] thymidine incorporation on day 3 from the start of centrifugation, and gradually decreased with culture up to day 9. Alkaline phosphatase activity was not affected by centrifugal force until day 5, and increased rapidly after day 7. Stimulation of DNA synthesis by centrifugation was abolished in the presence of H-7, an inhibitor of protein kinase C. These results suggest that centrifugal force stimulates the proliferation of osteoblastic cells through an autocrine secretion of some diffusable growth-promoting activity. Additional centrifugation of the cells also slightly stimulated alkaline phosphatase activity, although this did not directly influence the cell's osteocalcin-production activity. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 405~410 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Alkaline Phosphatase/metabolism | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Division | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Centrifugation | - |
dc.subject.MESH | DNA/biosynthesis | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Osteoblasts/physiology* | - |
dc.subject.MESH | Stress, Mechanical | - |
dc.title | Effect of Centrifugal Force on Cellular Activity of Osteoblastic MC3T3-E1 Cells in vitro | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학) | - |
dc.contributor.googleauthor | Dong Hee Lee | - |
dc.contributor.googleauthor | Jong Chul Park | - |
dc.contributor.googleauthor | Hwal Suh | - |
dc.identifier.doi | 10.3349/ymj.2001.42.4.405 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01662 | - |
dc.contributor.localId | A01924 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 11519082 | - |
dc.subject.keyword | Centrifugation | - |
dc.subject.keyword | osteoblast | - |
dc.subject.keyword | MC3T3-E1 | - |
dc.subject.keyword | alkaline phosphatase | - |
dc.subject.keyword | DNA synthesis | - |
dc.subject.keyword | fetal bovin serum | - |
dc.contributor.alternativeName | Park, Jong Chul | - |
dc.contributor.alternativeName | Suh, Hwal | - |
dc.contributor.affiliatedAuthor | Park, Jong Chul | - |
dc.contributor.affiliatedAuthor | Suh, Hwal | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 42 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 405 | - |
dc.citation.endPage | 410 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.42(4) : 405-410, 2001 | - |
dc.identifier.rimsid | 39101 | - |
dc.type.rims | ART | - |
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