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Effects of Whole Cell Sonicates of Treponema lecithinolyticum on Osteoclast Differentiation

Authors
 Bong-Kyu Choi  ;  Seung-Ho Ohk  ;  Hyun Jung Lee  ;  Jung Hwa Kang  ;  Gook Jin Jeong  ;  Yun-Jung Yoo 
Citation
 JOURNAL OF PERIODONTOLOGY, Vol.72(9) : 1172-1177, 2001 
Journal Title
JOURNAL OF PERIODONTOLOGY
ISSN
 0022-3492 
Issue Date
2001
MeSH
Alveolar Bone Loss/microbiology* ; Animals ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Bone Marrow Cells/drug effects ; Carrier Proteins/metabolism ; Cell Differentiation/drug effects ; Coculture Techniques ; Culture Media, Conditioned/metabolism ; Culture Media, Conditioned/pharmacology ; Dinoprostone/antagonists & inhibitors ; Dose-Response Relationship, Drug ; Indomethacin/pharmacology ; Membrane Glycoproteins/metabolism ; Mice ; Mice, Inbred ICR ; Osteoclasts/drug effects* ; Prostaglandin Antagonists/pharmacology ; RANK Ligand ; Receptor Activator of Nuclear Factor-kappa B ; Sonication ; Statistics, Nonparametric ; Treponema/pathogenicity*
Keywords
Alveolar bone loss/microbiology ; osteoclasts/growth ; periodontal diseases/microbiology ; Treponema lecithinolyticum
Abstract
BACKGROUND:
Alveolar bone destruction is a characteristic feature of periodontal diseases and multinucleated osteoclast cells derived from hemopoietic cells are responsible for bone resorption. Treponema lecithinolyticum is a novel oral spirochete isolated from the periodontal lesions.
METHODS:
The effect of whole cell sonicates on the osteoclast differentiation was examined in a co-culture system of hemopoietic mouse bone marrow cells and calvaria derived-osteoblastic cells to clarify the role of T. lecithinolyticum in the alveolar bone destruction associated with periodontal diseases. The differentiated osteoclasts were confirmed by tartrate-resistant acid phosphatase (TRAP) staining.
RESULTS:
Sonicates of this bacterium stimulated the osteoclast formation in the co-culture system in a dose-dependent manner. The sonicates-induced osteoclast formation was partially inhibited by the heat treatment of sonicates. Indomethacin, which is a prostaglandin inhibitor, decreased the osteoclast formation induced by the bacterial sonicates.
CONCLUSIONS:
These findings suggest that T. lecithinolyticum induces osteoclast differentiation by a prostaglandin E2-dependent mechanism and that heat-labile components may be involved in this process.
Full Text
http://www.joponline.org/doi/abs/10.1902/jop.2000.72.9.1172
DOI
10.1902/jop.2000.72.9.1172
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143124
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