Cited 0 times in
키토산이 치주인대 섬유아세포에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김종관 | - |
dc.contributor.author | 유윤정 | - |
dc.contributor.author | 조규성 | - |
dc.contributor.author | 최성호 | - |
dc.date.accessioned | 2016-02-19T11:25:35Z | - |
dc.date.available | 2016-02-19T11:25:35Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0250-3352 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143117 | - |
dc.description.abstract | Periodontal therapy has dealt primarily with attempts at arresting progression of disease, however, more recent techniques have focused on regenerating the periodontal ligament having the capacity to regenerate the periodontium. The effect of chitosan(poly-N-acetyl glucosaminoglycan), a carbohydrate biopolymer extracted from chitin, on periodontal ligament regeneration is of particular interest. The purpose of this study was to evaluate the effect of chitosan on the human periodontal ligament fibroblasts(hPDLFs) in vitro, with special focus on their proliferative properties by M'IT assay, the synthesis of type I collagen by reverse transcription-polymerase chain reaction(RT-PCR) and the activity of alkaline phosphatase(ALP). Fibroblast populations were obtained from individuals with a healthy periodontium and cultured with αMEM as the control group. The experimental groups were cultured with chitosan in concentration of 0.01,0.1, 1,2mg/ml. The results are as follows; 1. Chitosan-induced proliferative responses of hPDLFs reached a plateau at the concentration of O.lmg/ml(p<0.05). 2. When hPDLFs were stimulated with 0.lmg/ml chitosan, mRNA expression of type I collagen was up-regulated. 3. When hPDLFs were stimulated with 0.lmg/ml chitosan, ALP activity was significantly up-regulated(p<0.05). In summary, chitosan(0.lmg/ml) enhanced the type I collagen synthesis in the early stage, and afterwards, facilitated differentiation into osteogenic cells. The results of this in vitro experiment suggest that chitosan potentiates the differentiation of osteoprogenitor cells and may facilitate the formation of bone. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 823~832 | - |
dc.relation.isPartOf | Journal of Korean Academy of Periodontology (대한치주과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 키토산이 치주인대 섬유아세포에 미치는 영향 | - |
dc.title.alternative | The effects of chitosan on the human periodontal ligament fibroblasts in vitro | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | 백정원 | - |
dc.contributor.googleauthor | 이현정 | - |
dc.contributor.googleauthor | 유윤정 | - |
dc.contributor.googleauthor | 조규성 | - |
dc.contributor.googleauthor | 김종관 | - |
dc.contributor.googleauthor | 최성호 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02490 | - |
dc.contributor.localId | A03810 | - |
dc.contributor.localId | A04081 | - |
dc.contributor.localId | A00914 | - |
dc.relation.journalcode | J01492 | - |
dc.contributor.alternativeName | Kim, Chong Kwan | - |
dc.contributor.alternativeName | Yoo, Yun Jung | - |
dc.contributor.alternativeName | Cho, Kyoo Sung | - |
dc.contributor.alternativeName | Choi, Seong Ho | - |
dc.contributor.affiliatedAuthor | Yoo, Yun Jung | - |
dc.contributor.affiliatedAuthor | Cho, Kyoo Sung | - |
dc.contributor.affiliatedAuthor | Choi, Seong Ho | - |
dc.contributor.affiliatedAuthor | Kim, Chong Kwan | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 31 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 823 | - |
dc.citation.endPage | 832 | - |
dc.identifier.bibliographicCitation | Journal of Korean Academy of Periodontology (대한치주과학회지), Vol.31(4) : 823-832, 2001 | - |
dc.identifier.rimsid | 39043 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.