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마우스의 대뇌조직에서 방사선에 의한 아포토시스와 세포주기의 조절

Other Titles
 Regulation of Apoptosis and Cell Cycle in Irradiated Mouse Brain 
Authors
 오원용  ;  송미희  ;  정은지  ;  성진실  ;  서창옥 
Citation
 Journal of the Korean Society for Therapeutic Radiology and Oncology, Vol.19(2) : 146-152, 2001 
Journal Title
Journal of the Korean Society for Therapeutic Radiology and Oncology(대한방사선종양학회지)
ISSN
 1229-8719 
Issue Date
2001
Keywords
마우스 대뇌 방사선조사 ; 아포토시스 유도 ; 세포주기의 조절
Abstract
Purpose: To investigate the regulation of apoptosis and cell cycle in mouse brain irradiation. Materials and Methods: 8-week old male mice, C57B1/6J were given whole body γ -radiation with a single dose of 25 Gy using Cobalt 60 irradiator. At different times 1, 2, 4, 8 and 24hr after irradiation, mice were killed and brain tissues were collected. Apoptotic cells were scored by TUNEL assay. Expression of p53, Bcl-2, and Bax and cell cycle regulating molecules; cyclins B1, D1, E and cdk2, cdk4, p34cdc2 were analysed by Western blotting. Cell cycle was analysed by Flow cytometry. Results: The peak of radiation induced apoptosis is shown at 8 hour after radiation. With a single 25 Gy irradiation, the peak of apoptotic index in C57B1/6J is 24.0±0.25 (p<0.05) at 8 hour after radiation. Radiation upregulated the expression of p53/tubulin, Bax/tubulin, and Bcl-2/tubulin with 1.3, 1.1 and 1.45 fold increase, respectively were shown at the peak level at 8 hour after radiation. The levels of cell cycle regulating molecules after radiation are not changed significantly except cyclin D1 with 1.3 fold increase. Fractions of Go-G1, G2-M and S phase in the cell cycle does not specific changes by time. Conclusions: In mouse brain tissue, radiation induced apoptosis is particularly shown in a specific area, subependyma. These results and lack of radiation induced changes in cell cycle offer better understanding of radiation response of normal brain tissue
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Seong, Jin Sil(성진실) ORCID logo https://orcid.org/0000-0003-1794-5951
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143035
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