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마우스의 대뇌조직에서 방사선에 의한 아포토시스와 세포주기의 조절
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 성진실 | - |
dc.date.accessioned | 2016-02-19T11:23:19Z | - |
dc.date.available | 2016-02-19T11:23:19Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1229-8719 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143035 | - |
dc.description.abstract | Purpose: To investigate the regulation of apoptosis and cell cycle in mouse brain irradiation. Materials and Methods: 8-week old male mice, C57B1/6J were given whole body γ -radiation with a single dose of 25 Gy using Cobalt 60 irradiator. At different times 1, 2, 4, 8 and 24hr after irradiation, mice were killed and brain tissues were collected. Apoptotic cells were scored by TUNEL assay. Expression of p53, Bcl-2, and Bax and cell cycle regulating molecules; cyclins B1, D1, E and cdk2, cdk4, p34cdc2 were analysed by Western blotting. Cell cycle was analysed by Flow cytometry. Results: The peak of radiation induced apoptosis is shown at 8 hour after radiation. With a single 25 Gy irradiation, the peak of apoptotic index in C57B1/6J is 24.0±0.25 (p<0.05) at 8 hour after radiation. Radiation upregulated the expression of p53/tubulin, Bax/tubulin, and Bcl-2/tubulin with 1.3, 1.1 and 1.45 fold increase, respectively were shown at the peak level at 8 hour after radiation. The levels of cell cycle regulating molecules after radiation are not changed significantly except cyclin D1 with 1.3 fold increase. Fractions of Go-G1, G2-M and S phase in the cell cycle does not specific changes by time. Conclusions: In mouse brain tissue, radiation induced apoptosis is particularly shown in a specific area, subependyma. These results and lack of radiation induced changes in cell cycle offer better understanding of radiation response of normal brain tissue | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 146~152 | - |
dc.language | Korean | - |
dc.publisher | 대한방사선종양학회 | - |
dc.relation.isPartOf | Journal of the Korean Society for Therapeutic Radiology and Oncology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 마우스의 대뇌조직에서 방사선에 의한 아포토시스와 세포주기의 조절 | - |
dc.title.alternative | Regulation of Apoptosis and Cell Cycle in Irradiated Mouse Brain | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | 오원용 | - |
dc.contributor.googleauthor | 송미희 | - |
dc.contributor.googleauthor | 정은지 | - |
dc.contributor.googleauthor | 성진실 | - |
dc.contributor.googleauthor | 서창옥 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01956 | - |
dc.relation.journalcode | J01857 | - |
dc.subject.keyword | 마우스 대뇌 방사선조사 | - |
dc.subject.keyword | 아포토시스 유도 | - |
dc.subject.keyword | 세포주기의 조절 | - |
dc.contributor.alternativeName | Seong, Jin Sil | - |
dc.contributor.affiliatedAuthor | Seong, Jin Sil | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 19 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 146 | - |
dc.citation.endPage | 152 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society for Therapeutic Radiology and Oncology, Vol.19(2) : 146-152, 2001 | - |
dc.identifier.rimsid | 38966 | - |
dc.type.rims | ART | - |
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