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마우스의 대뇌조직에서 방사선에 의한 아포토시스와 세포주기의 조절

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dc.contributor.author성진실-
dc.date.accessioned2016-02-19T11:23:19Z-
dc.date.available2016-02-19T11:23:19Z-
dc.date.issued2001-
dc.identifier.issn1229-8719-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143035-
dc.description.abstractPurpose: To investigate the regulation of apoptosis and cell cycle in mouse brain irradiation. Materials and Methods: 8-week old male mice, C57B1/6J were given whole body γ -radiation with a single dose of 25 Gy using Cobalt 60 irradiator. At different times 1, 2, 4, 8 and 24hr after irradiation, mice were killed and brain tissues were collected. Apoptotic cells were scored by TUNEL assay. Expression of p53, Bcl-2, and Bax and cell cycle regulating molecules; cyclins B1, D1, E and cdk2, cdk4, p34cdc2 were analysed by Western blotting. Cell cycle was analysed by Flow cytometry. Results: The peak of radiation induced apoptosis is shown at 8 hour after radiation. With a single 25 Gy irradiation, the peak of apoptotic index in C57B1/6J is 24.0±0.25 (p<0.05) at 8 hour after radiation. Radiation upregulated the expression of p53/tubulin, Bax/tubulin, and Bcl-2/tubulin with 1.3, 1.1 and 1.45 fold increase, respectively were shown at the peak level at 8 hour after radiation. The levels of cell cycle regulating molecules after radiation are not changed significantly except cyclin D1 with 1.3 fold increase. Fractions of Go-G1, G2-M and S phase in the cell cycle does not specific changes by time. Conclusions: In mouse brain tissue, radiation induced apoptosis is particularly shown in a specific area, subependyma. These results and lack of radiation induced changes in cell cycle offer better understanding of radiation response of normal brain tissue-
dc.description.statementOfResponsibilityopen-
dc.format.extent146~152-
dc.languageKorean-
dc.publisher대한방사선종양학회-
dc.relation.isPartOfJournal of the Korean Society for Therapeutic Radiology and Oncology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title마우스의 대뇌조직에서 방사선에 의한 아포토시스와 세포주기의 조절-
dc.title.alternativeRegulation of Apoptosis and Cell Cycle in Irradiated Mouse Brain-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiation Oncology (방사선종양학)-
dc.contributor.googleauthor오원용-
dc.contributor.googleauthor송미희-
dc.contributor.googleauthor정은지-
dc.contributor.googleauthor성진실-
dc.contributor.googleauthor서창옥-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01956-
dc.relation.journalcodeJ01857-
dc.subject.keyword마우스 대뇌 방사선조사-
dc.subject.keyword아포토시스 유도-
dc.subject.keyword세포주기의 조절-
dc.contributor.alternativeNameSeong, Jin Sil-
dc.contributor.affiliatedAuthorSeong, Jin Sil-
dc.rights.accessRightsfree-
dc.citation.volume19-
dc.citation.number2-
dc.citation.startPage146-
dc.citation.endPage152-
dc.identifier.bibliographicCitationJournal of the Korean Society for Therapeutic Radiology and Oncology, Vol.19(2) : 146-152, 2001-
dc.identifier.rimsid38966-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

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