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3차원적 자궁내막 세포배양 체계의 확립 : 성선 호르몬에 의한 분화유도 및 특성

Other Titles
 Establishment of Three Dimensional in-vitro Culture System with Human Endometrial Cells : Induction of Differentiation by Sex Steroid Hormone & Characterization 
 권혁찬  ;  박원일  ;  김선영  ;  박동욱  ;  김미란  ;  한성원  ;  김대운  ;  이호준  ;  홍서유  ;  박준영  ;  김세광  ;  조동제 
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지), Vol.44(1) : 65-73, 2001 
Journal Title
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지) 
Issue Date
three dimensional co-culture ; human ednometrial cell ; integrin ; MMPs
Objective : The aim of this study was to establish three-dimensionally cultured endometrial cell model containing endometrial stromal cell (ESC), endometrial epithelial cell (EEC) and extracellular matrix (ECM) and to compare the morphological and biomolecular expression patterns of this model with mid-luteal endometrium in vivo. Materials and Methods : The EEC and ESC was obtained from hysterectomy specimen and cultured separately. The EEC was overlayered in Matrigel layer on ESC embedded in collagen. The model had been cultured for 48 h in DMEM medium containing estrogen and progesterone. The ultrastructure was evaluated by electron microscopy. The expression of integrins, cyclooxygenases and matrix metalloproteinases were examined by immunohistochemistry and zymography. Results : EEC in three-dimensional culture model grew with polarity and tight junction and desmosome between cells were found. The formation of pinopodes was also detected. In three-dimensionally cultured endometrial cell model, the expression of integrin α1, α4, β3, MMP-1, -2, -3 and 9 was detected which was not expressed in monolayer culture of EEC, ESC or ESC embedded in collagen. Conclusion : The three-dimensionally cultured endometrial cell model possessed the morphological and biomolecular characteristics of in vivo endometrium of implantation period. These characteristics could be achieved by paracrine interactions between ESC and EEC. This model may contribute to the studies of differentiation of endometrium, process of implantation and pathophysiology of implantation-related diseases.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sei Kwang(김세광)
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