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Evidence That Gal11 Protein Is a Target of the Gal4 Activation Domain in the Mediator

Other Titles
 Mediator 내에서 Gal11이 Gal4 AD의 표적이라는 증거 
Authors
 Choon-Ju Jeong  ;  Sang-Hwa Yang  ;  Yueqing Xie  ;  Lei Zhang  ;  Stephen Albert Johnston  ;  Thomas Kodadek 
Citation
 BIOCHEMISTRY, Vol.40(31) : 9421-9427, 2001 
Journal Title
 BIOCHEMISTRY 
ISSN
 0006-2960 
Issue Date
2001
MeSH
Binding, Competitive ; DNA-Binding Proteins ; Fungal Proteins/antagonists & inhibitors ; Fungal Proteins/genetics ; Fungal Proteins/metabolism* ; Fungal Proteins/physiology ; Mediator Complex ; Peptide Fragments/genetics ; Peptide Fragments/metabolism ; Protein Binding/genetics ; Protein Structure, Tertiary/genetics ; Recombinant Fusion Proteins/metabolism ; Repressor Proteins/metabolism ; Saccharomyces cerevisiae Proteins* ; Trans-Activators/genetics ; Trans-Activators/metabolism* ; Trans-Activators/physiology ; Transcription Factors/antagonists & inhibitors ; Transcription Factors/genetics ; Transcription Factors/metabolism*
Abstract
The mediator is an approximately 20 protein complex that is essential for the transcription of most genes in yeast. It is contacted by a number of gene-specific activators, but the details of these interactions are not well understood in most cases. Here, evidence is presented that the mediator component Gal11 represents at least one target of the Gal4 activation domain (AD). Deletion of Gal11 is shown to decrease the affinity of the Gal4 AD for the mediator, and direct binding of an N-terminal domain of Gal11 with the Gal4 AD is demonstrated. Quantitative studies, however, indicate that the KD of the 1:1 Gal4 AD−Gal11 complex is modest. Combined with in vivo data showing that Δgal11 cells exhibit reduced, but still significant, Gal4-mediated gene expression, these results suggest that the dimeric activator might also contact another protein in the mediator in addition to Gal11.
Full Text
http://pubs.acs.org/doi/abs/10.1021/bi010011k
DOI
10.1021/bi010011k
Appears in Collections:
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
Yonsei Authors
Yang, Sang Hwa(양상화)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142951
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