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Epitheliomesenchymal Transdifferentiation of Cultured RPE Cells

Authors
 Lee S.C.  ;  Kwon O.W.  ;  Seong G.J.  ;  Kim S.H.  ;  Ahn J.E.  ;  Kay E.D.P. 
Citation
 OPHTHALMIC RESEARCH, Vol.33(2) : 80-86, 2001 
Journal Title
 OPHTHALMIC RESEARCH 
ISSN
 0030-3747 
Issue Date
2001
MeSH
Actins/biosynthesis ; Animals ; Cell Differentiation/drug effects ; Cell Division/drug effects ; Cells, Cultured ; Collagen/biosynthesis ; Dose-Response Relationship, Drug ; Metaplasia ; Phenotype ; Pigment Epithelium of Eye/drug effects ; Pigment Epithelium of Eye/metabolism ; Pigment Epithelium of Eye/pathology* ; Swine ; Transforming Growth Factor beta/pharmacology ; Transforming Growth Factor beta2 ; Vitreoretinopathy, Proliferative/pathology
Keywords
Retinal pigment epithelial cells ; Proliferative vitreoretinopathy ; Collagen ; Transforming growth factor ; Transdifferentiation
Abstract
Retinal pigment epithelium (RPE) cells of the proliferative vitreoretinopathy (PVR) membrane take on the shape of fibroblasts and participate in fibrosis, thus deviating from the character of epithelial cells. This study was undertaken to evaluate RPE cell transdifferentiation in vitro. During the culture of porcine RPE cells, primary and 10th-passaged RPE cells were investigated for cell growth in response to transforming growth factor (TGF) β2, change of phenotype and amount in collagen synthesis as well as expression of α-smooth-muscle actin (α-SMA). TGF-β2 inhibited the proliferation of the primary cultures of RPE cells in a dose-dependent manner, while the spindle-shaped 10th-passaged RPE cells were not inhibited by TGF-β2. The 10th-subcultured cells did not show much difference in the quality of collagen synthesis, other than type VIII collagen which was not produced. Collagen synthesis was dose-dependently stimulated by TGF-β2. The stimulation by TGF-β2 in the 10th-passaged RPE cells was much greater than in primary RPE cells. The 10th-subcultured RPE cells produced substantial α-SMA compared to α-SMA production by primary RPE cells. These results were also observed by confocal laser microscopy. These findings indicated that RPE metaplasia resulting in a change of biological cell behavior might be a necessary predisposing step in the development of PVR.
Full Text
http://www.karger.com/Article/FullText/55648
DOI
10.1159/000055648
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Seong, Gong Je(성공제) ORCID logo https://orcid.org/0000-0002-5456-4296
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142823
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