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A Novel Recombinant Basic Fibroblast Growth Factor and Its Secretion

Authors
 Young-Doug Sohn  ;  Hyun Joung Lim  ;  Ki-Chul Hwang  ;  Jun Hye Kwon  ;  Hyun-Young Park  ;  Kwang-Hoe Chung  ;  Seung Yun Cho  ;  Yangsoo Jang 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.284(4) : 931-936, 2001 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2001
MeSH
3T3 Cells ; Animals ; Aorta, Thoracic/cytology ; Aorta, Thoracic/drug effects ; Aorta, Thoracic/physiology ; COS Cells ; Cell Division/drug effects ; Cells, Cultured ; Cercopithecus aethiops ; Cloning, Molecular/methods ; DNA/biosynthesis ; Dose-Response Relationship, Drug ; Fibroblast Growth Factor 2/biosynthesis ; Fibroblast Growth Factor 2/genetics* ; Fibroblast Growth Factor 2/pharmacology ; Fibroblast Growth Factor 4 ; Fibroblast Growth Factors/genetics ; Fibroblast Growth Factors/pharmacology ; Fibroblasts/metabolism ; Humans ; Mice ; Muscle, Smooth, Vascular/cytology* ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/physiology* ; Polymerase Chain Reaction ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins/pharmacology ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins/pharmacology ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; Skin/metabolism ; Transfection/methods
Keywords
basic fibroblast growth factor ; recombinant ; secretion
Abstract
Basic fibroblast growth factor (FGF-2) is a pleiotropic mitogen which plays an important role in cell growth, differentiation, migration, and survival in different cells and organ systems. Recently, several clinical applications for FGF-2 gene transfer are being evaluated in wound healing and collateral artery development to relieve myocardial and peripheral ischemia due to the ability of FGF-2 to regulate the growth and function of vascular cells. However, FGF-2 lacks a classical hydrophobic secretion signal peptide, the FGF-2 chimeras containing various signal sequences have been explored. In this study, a novel recombinant 4sFGF-2 was constructed by replacing nine residues from the amino-terminus of native FGF-2 (Met1 to Leu9) with eight amino acid residues of signal peptide of FGF-4 (Met1 to Ala8) to better increase the secretion level of FGF-2. When the recombinant FGF-2 gene, cloned into the expression vector with CMV promoter, was expressed in COS-7 cells, the recombinant 4sFGF-2 was highly secreted into the media. The secreted 4sFGF-2 showed the same biological activity as the native FGF-2 in the dose-response effects on DNA synthesis and cell growth of rat aortic smooth muscle cells (RASMCs) and NIH3T3 cells. The 4sFGF-2 also was able to activate MAPK as wild FGF-2 in RASMCs. These results indicate that a novel recombinant 4sFGF-2 may be useful as clinical applicability of angiogenic growth factor gene transfer.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X01950764?np=y
DOI
10.1006/bbrc.2001.5076
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142752
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