Cited 21 times in
A Novel Recombinant Basic Fibroblast Growth Factor and Its Secretion
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 장양수 | - |
dc.contributor.author | 황기철 | - |
dc.date.accessioned | 2016-02-19T11:15:42Z | - |
dc.date.available | 2016-02-19T11:15:42Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142752 | - |
dc.description.abstract | Basic fibroblast growth factor (FGF-2) is a pleiotropic mitogen which plays an important role in cell growth, differentiation, migration, and survival in different cells and organ systems. Recently, several clinical applications for FGF-2 gene transfer are being evaluated in wound healing and collateral artery development to relieve myocardial and peripheral ischemia due to the ability of FGF-2 to regulate the growth and function of vascular cells. However, FGF-2 lacks a classical hydrophobic secretion signal peptide, the FGF-2 chimeras containing various signal sequences have been explored. In this study, a novel recombinant 4sFGF-2 was constructed by replacing nine residues from the amino-terminus of native FGF-2 (Met1 to Leu9) with eight amino acid residues of signal peptide of FGF-4 (Met1 to Ala8) to better increase the secretion level of FGF-2. When the recombinant FGF-2 gene, cloned into the expression vector with CMV promoter, was expressed in COS-7 cells, the recombinant 4sFGF-2 was highly secreted into the media. The secreted 4sFGF-2 showed the same biological activity as the native FGF-2 in the dose-response effects on DNA synthesis and cell growth of rat aortic smooth muscle cells (RASMCs) and NIH3T3 cells. The 4sFGF-2 also was able to activate MAPK as wild FGF-2 in RASMCs. These results indicate that a novel recombinant 4sFGF-2 may be useful as clinical applicability of angiogenic growth factor gene transfer. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 931~936 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | 3T3 Cells | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Aorta, Thoracic/cytology | - |
dc.subject.MESH | Aorta, Thoracic/drug effects | - |
dc.subject.MESH | Aorta, Thoracic/physiology | - |
dc.subject.MESH | COS Cells | - |
dc.subject.MESH | Cell Division/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cercopithecus aethiops | - |
dc.subject.MESH | Cloning, Molecular/methods | - |
dc.subject.MESH | DNA/biosynthesis | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Fibroblast Growth Factor 2/biosynthesis | - |
dc.subject.MESH | Fibroblast Growth Factor 2/genetics* | - |
dc.subject.MESH | Fibroblast Growth Factor 2/pharmacology | - |
dc.subject.MESH | Fibroblast Growth Factor 4 | - |
dc.subject.MESH | Fibroblast Growth Factors/genetics | - |
dc.subject.MESH | Fibroblast Growth Factors/pharmacology | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Muscle, Smooth, Vascular/cytology* | - |
dc.subject.MESH | Muscle, Smooth, Vascular/drug effects | - |
dc.subject.MESH | Muscle, Smooth, Vascular/physiology* | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Proto-Oncogene Proteins/genetics | - |
dc.subject.MESH | Proto-Oncogene Proteins/pharmacology | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Recombinant Fusion Proteins/pharmacology | - |
dc.subject.MESH | Recombinant Proteins/biosynthesis | - |
dc.subject.MESH | Recombinant Proteins/pharmacology | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Skin/metabolism | - |
dc.subject.MESH | Transfection/methods | - |
dc.title | A Novel Recombinant Basic Fibroblast Growth Factor and Its Secretion | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Young-Doug Sohn | - |
dc.contributor.googleauthor | Hyun Joung Lim | - |
dc.contributor.googleauthor | Ki-Chul Hwang | - |
dc.contributor.googleauthor | Jun Hye Kwon | - |
dc.contributor.googleauthor | Hyun-Young Park | - |
dc.contributor.googleauthor | Kwang-Hoe Chung | - |
dc.contributor.googleauthor | Seung Yun Cho | - |
dc.contributor.googleauthor | Yangsoo Jang | - |
dc.identifier.doi | 10.1006/bbrc.2001.5076 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03448 | - |
dc.contributor.localId | A04456 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 11409882 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X01950764?np=y | - |
dc.subject.keyword | basic fibroblast growth factor | - |
dc.subject.keyword | recombinant | - |
dc.subject.keyword | secretion | - |
dc.contributor.alternativeName | Jang, Yang Soo | - |
dc.contributor.alternativeName | Hwang, Ki Chul | - |
dc.contributor.affiliatedAuthor | Jang, Yang Soo | - |
dc.contributor.affiliatedAuthor | Hwang, Ki Chul | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 284 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 931 | - |
dc.citation.endPage | 936 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.284(4) : 931-936, 2001 | - |
dc.identifier.rimsid | 29819 | - |
dc.type.rims | ART | - |
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