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Comparison of allergenic components between German cockorach whole body and fecal extract

 Yeong-Yeon Yun  ;  Si H. Ko  ;  Jung Won Park  ;  In-Yong Lee  ;  Han Il Ree  ;  Chein-Soo Hong 
 ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, Vol.86(5) : 551-556, 2001 
Journal Title
Issue Date
Allergens/immunology ; Allergens/isolation & purification* ; Animals ; Antibodies, Monoclonal/immunology ; Antibody Specificity ; Antigens, Plant ; Aspartic Acid Endopeptidases/immunology ; Aspartic Acid Endopeptidases/isolation & purification ; Asthma/blood ; Asthma/immunology ; Blotting, Western ; Cockroaches/chemistry ; Cockroaches/immunology* ; Cross Reactions ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Feces/chemistry* ; Humans ; Immunoglobulin E/immunology ; Insect Proteins/immunology ; Insect Proteins/isolation & purification* ; Mites/immunology ; Rhinitis, Allergic, Perennial/blood ; Rhinitis, Allergic, Perennial/immunology ; Skin Tests ; Tissue Extracts/analysis ; Tissue Extracts/immunology
BACKGROUND: Cockroaches have been demonstrated to be an etiologic factor in allergic diseases. Further, sensitivity to cockroach places patients with asthma at risk for exacerbations that require emergency medical care. OBJECTIVE: This study compared the differences in allergenic components between German cockroach whole body and German cockroach fecal extracts (GWBE and GFE). METHODS: Patients with asthma and/or allergic rhinitis were skin prick tested with German cockroach extract (Bayer Corporation, West Haven, CT). Serum specimens from these patients, 25 with positive skin tests and 8 with negative tests, were used for the ELISA and immunoblot experiments. RESULTS: By ELISA, 72% (18 of 25) and 60% (15 of 25) of positive responders' sera showed IgE antibodies to GWBE and GFE, respectively, and the IgE levels to GWBE were highly correlated with those to GFE (r = .84, P < .01). In inhibition ELISA experiments, extensive cross-reactivity was observed between GWBE and GFE, slight cross-reactivity between GWBE and Dermatophagoides farinae, and no cross-reactivity between GFE and D. farinae. The two-site monoclonal antibody ELISA detected more of the German cockroach major allergens in GFE compared with GWBE; 6.2 times (2420 vs 390 U/mL) for Bla g 1 and 3 times (15.32 vs 5.07 microg/mL) for Bla g 2. In the immunoblot comparison of patients' sera, the IgE antibodies binding to GWBE were apparently different from those binding to GFE in all the positive responders' sera; eg, 50% or more of the 25 positive responders' sera reacted to 43- to 67-kDa proteins in GWBE and to 28- to 30-kDa proteins in GFE, respectively. No IgE antibodies bound to components in GWBE and GFE in the 8 negative responders' sera. CONCLUSIONS: There are major differences between the allergenic components of GWBE and GFE. Based on the amounts of major allergens (Bla g 1, Bla g 2), German cockroach feces are a more important source of allergen than the whole body in respiratory allergic diseases.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
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